臺灣博碩士論文加值系統

為瞭解豬放線桿菌胸膜肺炎多形細胞之來源及特性，本實驗以胸膜肺炎放
線桿菌接種無特異病原豬隻後,觀察接種細菌後第3、6、9、12、 15、18
、24及48小時，豬肺泡腔內細胞形態之變化，並與臨床病例相比較；同時
以酵素細胞化學染色及穿透式電子顯微鏡，鑑定這些多形細胞。此外，並
以敗血性巴氏桿菌接種無特異病原豬隻，觀察其肺泡腔內細胞形態之變化
，並與胸膜肺炎放線桿菌之病例相比較。實驗結果顯示，在感染胸膜肺炎
放線桿菌之初期，豬隻肺泡腔內以嗜中性球聚積為主，但感染24小時以後
，多形細胞便成為本病最顯著的細胞形態。而感染敗血性巴氏桿菌之豬隻
，肺泡腔內皆以嗜中性球浸潤為主。豬放線桿菌胸膜肺炎之多形細胞經酵
素細胞化學染色及穿透式電子顯微鏡鑑定後，證實來源不只一種，巨噬細
胞及第二型肺泡上皮細胞均有可能。

The polymorphic cells, are usually in the pneumonic lesions
of Actinobacillus pleuropneumoniae infected pigs.
Specific pathogen free pigs were inoculated intratracheally
with Actino- bacillus pleuropneumoniae, in order to understand
the origin and characteristics of these cells. The pigs were
sacrificed at 3, 6 , 9, 12, 15, 18, 24 and 48 hours after
inoculation. The cells in the alveolar spaces were observed
with light and electron micro- scopes, and analyzed with enzyme
cytochemistry. The differences of the cells in the alveolar
spaces induced by Actionbacillus pleuropneumoniae and
Pasterella multocida were also compared. The results indicated
that a lot of neutrophils were observed in the alveolar spaces
in the early stage after Actinobacillus pleuropneumoniae
inoculation. Twenty four hours later, polymor- phic cells
appeared abundantly. Contrarily, the cells in the alveolar
spaces of pneumonic lesions induced by Pasteurella mul- tocida
were mainly neutrophils. By enzyme cytochemical and elec- tron
microscopic examination, the polymorphic cells were found
that these cells were derived from macrophages and type
Ⅱ alveolar epithelial cells.