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Boldenone undecylenate (1.1 mg/kg) was injected I.M. to
three geldings (one half-thoroughbred and two ponies) and two
mares (palomino and thoroughbred). Blood and urine samples were
collected for a period of 8 weeks. Levels of boldenone and
furosemide were determined by ELISA kits. Results showed that
serum had a mean level of peak of 1.39 ± 0.17 ng/ml of
boldenone at 4 hours of administration and gradually decreased
to undetectable levels at 5 weeks. The highest urinary level
of boldenone was 1.95 ± 0.23 ng/ml at 6 hours and gradually
decreased to undetectable levels at 6 weeks. In another trial,
furosemide (1.0 mg/kg) was injected I.V. 7 days following
boldenone administration. Results showed that urinary levels of
boldenone were significantly decreased by furosemide as a
result of diuresis. The levels of boldenone were below 0.02 ng/
ml, even 0 ng/ml, for the first 4 hours following
furosemide administration, and began to increase at 4 hours,
and rose to 0.22 ± 0.07 ng/ml at 6 hours. However,
furosemide had no significant effect on serum levels of
boldenone. A trial was conducted to assess the effect of
exercise on the concentration and clearance time of boldenone
in equine serum and urine. The horses were trotted 20 minutes
at a speed of 200 meters a miniute 7 days following boldenone
administra- tion. Results showed that exercise did not cause
significant difference on serum or urinary levels of
boldenone from boldenone administration alone. In another
trial, urinary levels of boldenone were significantly
decreased by furosemide when furosemide was injected I.V.
30 minutes following trotting at the same speed.
However, the result had no significant difference from
the combination injection of boldenone and furosemide.
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