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Boldenone undecylenate (1.1 mg/kg) was injected I.M. to three geldings (one half-thoroughbred and two ponies) and two mares (palomino and thoroughbred). Blood and urine samples were collected for a period of 8 weeks. Levels of boldenone and furosemide were determined by ELISA kits. Results showed that serum had a mean level of peak of 1.39 ± 0.17 ng/ml of boldenone at 4 hours of administration and gradually decreased to undetectable levels at 5 weeks. The highest urinary level of boldenone was 1.95 ± 0.23 ng/ml at 6 hours and gradually decreased to undetectable levels at 6 weeks. In another trial, furosemide (1.0 mg/kg) was injected I.V. 7 days following boldenone administration. Results showed that urinary levels of boldenone were significantly decreased by furosemide as a result of diuresis. The levels of boldenone were below 0.02 ng/ ml, even 0 ng/ml, for the first 4 hours following furosemide administration, and began to increase at 4 hours, and rose to 0.22 ± 0.07 ng/ml at 6 hours. However, furosemide had no significant effect on serum levels of boldenone. A trial was conducted to assess the effect of exercise on the concentration and clearance time of boldenone in equine serum and urine. The horses were trotted 20 minutes at a speed of 200 meters a miniute 7 days following boldenone administra- tion. Results showed that exercise did not cause significant difference on serum or urinary levels of boldenone from boldenone administration alone. In another trial, urinary levels of boldenone were significantly decreased by furosemide when furosemide was injected I.V. 30 minutes following trotting at the same speed. However, the result had no significant difference from the combination injection of boldenone and furosemide.
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