(3.230.76.48) 您好!臺灣時間:2021/04/15 00:29
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:陳泰嬌
研究生(外文):Chen, Tai-Jiao
論文名稱:藍藻Anabasmasp.CH1及CH3在不同氮源下之氮代謝途徑
論文名稱(外文):Nitrogen assimilation of anabaena sp. CH1 and CH3 in differant nitrogen souroes
指導教授:陳伯中陳伯中引用關係
指導教授(外文):Chen, Bo-Zhong
學位類別:碩士
校院名稱:國立中興大學
系所名稱:植物學研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:1994
畢業學年度:82
語文別:中文
論文頁數:84
中文關鍵詞:藍藻氮源代謝途徑植物科學植物學異形細菌固氮作用氨化作用
外文關鍵詞:CH1CH3nitrogensourcesPLANT-SCIENCEBOTANYCyanobacteriumNitrogen FixationAmmonium-assimilation
相關次數:
  • 被引用被引用:0
  • 點閱點閱:77
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:1

藍藻Anabaena sp. CH1 及 CH3生長在氮氣的環境下,其固氮比活性分別
為17 mU/mg prot. 與24 mU/mg prot. 異形細胞頻率為 7.4 % 與 7.7%生
長速率為 0.083 h-1 與 0.089 h-1 生長加倍時間為 8 h 與 7 h ,及GS
酵素比活性為 507mU/mg prot. 與 592mU/mg prot.Anabaena sp. CH1
及 CH3 對 NH4+ 氮源相當地敏感, 不能在含 0.25 mM NH4Cl NH4NO3
(Anabaena CH1) 或0.5 mM NH4Cl NH4NO3 (Anabaena CH3) 的培養液中生
長.當生長在含 0.03-0.1 mM NH4+ 的培養液中,GOGAT 酵素比活性幾乎不
受NH4Cl NH4NO3 的影響,GS 酵素比活性下降約為氮氣培養時的 72-76
%, ADH 及GDH 酵素比活性分別提高約為氮氣培養時的 195- 199 % 與
257-260 %, 因此, 能在 0.05 mM NH4Cl 0.03 mM NH4NO3 (Anabaena
CH1)或 0.1 mM NH4Cl 0.05 mM NH4NO3 的培養液中生長 (Anabaena
CH3).相反地, Anabaena sp. CH1 及 CH3對 NO3- 忍受性很強,在含 30
50 mM KNO3之培養液中仍能維持良好的生長{0.081 h-1 0.054h-1
(Anabaena CH1);0.084 h-1 0.073h-1 (Anabaena CH3) } ,唯不能在濃
度 70 mM KNO3 以上的培養液中生長. Anabaena sp. CH1及 CH3 之固氮
比活性 生長速率 異形細胞頻率及 GS 比活性, 皆會隨 KNO3 NH4Cl 及
NH4NO3濃度的提高迅速地下降. Anabaena sp. CH1 及CH3 在固氮及氨化
作用的過程中, 皆需要能量的供應,經使用抑制劑間接測定的結果,其所需
的能源主要由光合作用所供給.

The highest of nitrogenase specific activity (17 mU/mg prot.
24mU/mg prot.) heterocyst frequency (7.4 % 7.7 %) growth rate
(0.083 h-1 0.089 h-1) doubling time (8 h 7 h) and GS specific
activity (507 mU/mg prot.592 mU/mg prot.) were obtained from
Anabaena sp. CH1 and CH3 grown in the presence of atmospheric
N2. Most of them more sensitive to NH4+, growth was inhibited
when the concentration higher than 0.25 mM NH4Cl NH4NO3
(Anabaena CH1) or 0.5 mM NH4Cl NH4NO3 (Anabaena CH3). When
Anabaena sp.CH1 and CH3 were grew in 0.03-0.1 mM NH4+-
containing media, the GOGAT specific activity was not affected
in the presence of KNO3 NH4Cl and NH4NO3. In addition, the GS
specific activity was 72-76 % as compared to that in N2-grown
cultures, and the ADH specific activity GDH specific activity
were stimulated 195-199 % 257-260 %. For these isolates grown
in the medium containing 0.05 mM NH4Cl 0.03 mM NH4NO3 (Anabaena
CH1)or 0.1 mM NH4Cl 0.05 mM NH4NO3 (Anabaena CH3 ).In contrast,
most of them were very insensitive to NO3-. These isolates grew
well in the medium containing KNO3 up to 30 50 mM{0.081 h-1
0.054 h-1(Anabaena CH1);0.084 h-1 0.073 h-1 (Anabaena CH3)} ,
but the growth were inhibited when the concentration higher
than 70 mM KNO3. However, with the increase of the KNO3 NH4Cl
and NH4NO3 concentration in medium, their nitrogenase specific
activity growth rate heterocyst frequency and GS specific
activity declined rapidly. Energy were needed for nitrogen
fixation and ammonium assimilation in Anabaena sp.CH1 and CH3,
The energy produced from photosynthesis was believed to be the
main source for nitrogen fixation and ammonium assimilation of
them.

QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關期刊
 
系統版面圖檔 系統版面圖檔