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The purposes of this study were:1)to establish an in vitro
model of bone formation, 2)to investigate the effects
of interleukin-1(IL-1 )on bone formation,3)to establish an in
vitro bioassay of osteoclast formation and 4)to clarify the
effects of IL-1 on bone resorption. Thirteen-fourteen day
BALB/C fetal calvarial cells were obtained and cultured for 3
weeks.With light microscopic observation, quantitation of bone
colony and alkaline phosphatase assay, an in vitro model of
bone formation could be established. By this model, daily
adding of IL-1 was performed during culture of fetal
calvarial cells.The effect of IL-1 on bone formation was
evaluated using the same methods mentioned above.In
addition, 14-day fetal calvarial explant was obtained and
cultured for 10 days. Light density mononuclear cells were
collected and co- cultured with bone explant.Ten day
after co-culture, osteoclasts were formed. By this model,IL-1
was added daily during co-culture.Ten days later, the
precise effect of IL-1 on bone resorption was evaluated.The
results of this study indicated that:1)a new in vitro model
of bone formation was established,2)there was a positive
correlation between the number of plated cells and the number
of bone clolnies,3)IL-1 exerted a potent dose-dependent
inhibition on bone formation,4)a suitable in vitro model of
osteoclast formation was established, 5)IL-1 could stimulate
bone resorption in a dose- related fashion,6)the increase in
the number of osteo- clasts was probably caused by
stimulating the fusion of mononuclasr cells and 7)IL-1 could
act "uncoupling factor" in bone remodeling process.
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