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研究生:黃雅淑
研究生(外文):Hwang,Yea-Shwu
論文名稱:限制活動壓力對雄性大花鼠的行為及相關腦區Monoamine含量的影響
論文名稱(外文):Effects of Restraint Stress on Behavior and Brain Monoamine Concentrations in Male Rats
指導教授:蔡元奮蔡元奮引用關係
指導教授(外文):Tsai,Yuan-Feen
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:生理學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:1994
畢業學年度:82
語文別:中文
論文頁數:96
中文關鍵詞:限制活動年老動作量食量性行為
外文關鍵詞:Restraint StressAgingMotor ActivityFood IntakeSexual
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本研究以年齡為 4-5 個月(年輕組)及24-30個月(年老組)的Long-Evans
雄鼠為實驗動物,使其接受單次或連續 8 天,每天兩小時的限制活動之
壓力 (Restraint Stress)。結果發現單次的限制活動壓力明顯地影響年
輕和年老動物的動作量。然而,接受多次壓力後的 24小時,年輕組動物
的各項動作量並無明顯的改變,而年老動物除了直立次數呈現明顯增加外
,其他各項動作量同年輕組動物一樣不再有明顯的改變。多次壓力實驗期
間,年輕動物在壓力解除後 2小時內的食量呈現明顯的減少,但其解除壓力
後22小時內的食量卻未有顯著的改變。年老動物則在壓力解除後2小時或
22小時內,食量均呈明顯地下降。 在多次壓力實驗期間年輕和年老實驗組
動物的體重較其同年齡之控制組有顯著減少的現象。而年老動物接受多次
壓力後,腎上腺重量明顯增加。而此現象並未在年輕組動物身上發現。此
外,腦下腺重量和腦中 Catecholamine之含量,不論是年輕或年老動物均未
發現實驗組與控制組間有顯著的差異。 綜合上述結果顯示年老動物受到
壓力後行為改變的情況與年輕動物相似,但其對壓力的調節和適應的能力
卻明顯較年輕者為差。 Menendez-Patterson 等人(1980) 發現限制活動
壓力會造成 Wistar 雄鼠性行為的表現變差,但是迄今並不清楚此現象和
控制雄鼠性行為相關腦區內 Monoamine系統的關係。本研究以年齡3-4個
月的 Long-Evans雄鼠作為實驗動物,接受單次兩小時的限制活動後,立
刻進行30分鐘的性行為測試,或接受連續 8天,每天兩小時的限制活動後
,立刻或經過 24小時後再進行性行為的測試。所有動物在完成行為測試
後立刻斷頭犧牲,以高壓液相層析法( HPLC)分析腦中Monoamine 的含量
。結果發現不論是單次或多次的限制活動均不能造成雄鼠性行為明顯的改
變。然而分析動物腦中Monoamine含量卻發現接受單次限制活動的雄鼠,
其內視前區的Norepinephrine 含量呈現明顯的下降,而在紋狀體和黑質
兩處,5-Hydroxyindoleacetic Acid的含量則有明顯增加的現象。至於其
他腦區如依核、腹蓋區、嗅結節和終紋底核 均未發現Monoamine濃度有顯
著的改變。此外,雄鼠接受多次限制活動壓力並測試性行為後,立即犧牲
,其腦中Monoamine含量與控制組比較並無顯著的差異。但是若動物延
後24小時再予以犧牲,其內視前區的Norepinephrine 和嗅結節的
Dopamine 濃度卻明顯地高於控制組動物。本實驗結果顯示限制活動並不
能造成Long-Evans雄鼠性行為的改變。

The purpose of this study was to investigate the effects of re-
straint stress (RS) on behaviors and catecholamine (CA)
contents brain in young and old rats. Male Long-Evans rats,aged
4-5 months (young group) and 24-30 months (old group), were
used. Motor ac- tivities were tested after a single 2hr RS
immediately or 24 hours after daily exposure to a 2hr RS for 8
days. Food intake and body weight were measured daily during
repeated RS session. Rats were killed immediately after the
last motor activity test. Contents of CA in brain were assayed
by HPLC-EC detection. A single but not repeated RS reduced
motor activities in young and old rats. RS obviously decreased
daily food intake in old but not young rats. A remarkable loss
of body weight was observed in young and old rats following RS.
No marked changes in brain CA contents were found in either
young or old rats after repeated The results suggest that the
adaptation to stress is age-related. The aim of this study is
to investigate effects of RS on sexual behavior (SB) and
monoamine (MA) contents of brain in rats. Male Long-Evans rats
were assigned to receive 2hr RS either once or daily for 8
days. SB was tested after exposure to a single or re- peated
stress immediately or 24 hours following repeated stress. After
SB test, all animals were killed and their brains were re-
moved rapidly. MA contents in brain were assayed by HPLC-EC de-
tection. Single or repeated RS caused no marked changes in SB.
However, single stress induced norepinephrine (NE) content de-
crease in medial preoptic area (MPOA) and 5-hydroxyindole
acetic acid increase in striatum and substantia nigra. A marked
increase in NE contents in MPOA and dopamine levels in
olfactory tubercleurs after repeated stress. These results
suggest that RS may not induce SB changes in male Long- Evans
rats and the changes of MA contents in brain induced by RS may
be unrelated to sexual behavior in male rats.

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