跳到主要內容

臺灣博碩士論文加值系統

(44.201.97.224) 您好!臺灣時間:2024/04/18 02:03
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:黃于哲
研究生(外文):Huang, Yu-Jer
論文名稱:臺灣地區豬輪狀病毒的疫情調查
論文名稱(外文):A Survey of Swine Rotavirus Infections in Taiwan
指導教授:賴秀穗賴秀穗引用關係
指導教授(外文):Lai, Shiow-suey
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:獸醫學系
學門:獸醫學門
學類:獸醫學類
論文種類:學術論文
論文出版年:1994
畢業學年度:82
語文別:中文
論文頁數:108
中文關鍵詞:輪狀病毒調查流行病學臺灣
外文關鍵詞:RotavirusSurveyEpidemiologyTaiwan
相關次數:
  • 被引用被引用:1
  • 點閱點閱:349
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
本實驗係以反轉錄聚合脢鏈反應(Reverse Transcriptase / Polymerase
Chain Reaction , RT/PCR )的技術調查本省豬場內各階段豬隻感染輪狀
病毒的情形,調查對象包括自懷孕期及哺乳期的母豬、及種公豬及哺乳期
到肥育期的仔豬。本實驗共選擇台灣地區北、中、南三個豬場作為調查對
象,收集各類豬隻的糞便,以RT/PCR作檢測,並以電子顯微鏡(EM)檢測部
份檢體做為比較。同時並採集分娩前後的母豬血清,以間接酵素結合免疫
吸附法(Enzyme - Linked Immunosorbent Assay , ELISA)技術檢測輪狀
病毒抗體力價。本實驗所設計的引子進行RT/PCR時,除可診斷輪狀病毒感
染的檢體外同時可作血清定型。在野外糞材的檢測中,共收集1532個檢體
,檢測的結果中有598個陽性,佔39%,581個檢體屬第5型OSU株輪狀病毒
,5個檢體屬第4 型Gottfried株,其中有兩個檢體是 OSU及Gottfried株
混合感染,有14個檢體無法作血清分類。北、中、南三場的感染率相當接
近,分別為37.8, 41.9及37.2%;從不同階段的豬隻感染率分析:離乳期
仔豬最高,約65.5%;其次為種公豬及哺乳仔豬,分別為48.6及47.8%;以
哺乳期母豬感染率最低,僅22.8%。比較RT/PCR與 EM檢測糞材中輪狀病毒
的敏感性:131個檢體中RT/PCR陽性者有64 個 (48.9%),EM陽性者有17
個(13.0%),其中有6 個EM 陽性的檢體RT/PCR呈陰性。以ELISA檢測自九
個豬場所採集的153 頭分娩母豬的血清輪狀病毒抗體,結果高達100%的母
豬具有抗體,力價分佈自16到4096倍之間。

Reverse transcriptase / polymerase chain reaction (RT/PCR ) was
used to detect rotavirus in porcine feces collected from 3 pig
farms located in Northern, Middle and Southern part of Taiwan,
respectively. The fecal samples were collected from sows,
boars, suckling, weaned and fattenning pigs, respectively.
Serum samples from sows were also titrated for antibodies to
rotavirus by Enzyme - Linked Immunosorbent Assay (ELISA). Type
- specific primers were used to serotype OSU and Gottfried
strains of group A rotavirus. In this experiment, a total of
1532 fecal samples were examined by RT/PCR: 598 samples(39%)
were positive. Among 598 positive samples, 581 were type 5
(OSU); 5 were type 4 (Gottfried); 2 samples had mixed
infection with OSU and Gottfried strain. However, 14 samples
could not be typed. The situation of rotavirus infection at 3
farms was fairly similar. The percentages of positive rate
found in pig farms located in Northern, Middle and Southern
parts of Taiwan were 37.8%, 41.9% and 37.2% , respectively.
Results of rotavirus infection in different stages of pigs
showed that the weaned pigs had the highest rate(65.5%),
followed by boars(48.6%) and suckling piglets(47.8%). Sows had
the lowest rate of infection(22.8%). A total of 153 serum
samples of sows were all positive for antibody against
rotavirus. The ELISA antibody titers ranged from 1:16 to
1:4096. The sensitivities of RT/PCR and EM to detect rotavirus
in fecal samples was compared and discussed.

QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
無相關期刊