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研究生:范鸝婉
研究生(外文):Lir-Wan Fan
論文名稱:以植物組織培養法生產紫田薯花青素之研究
論文名稱(外文):Studies on Anthocyanin Production in Tissue Culture of Dioscorea alata L. var. purpurea M. Pouch
指導教授:溫銘■
指導教授(外文):Ming C. Wen
學位類別:碩士
校院名稱:東海大學
系所名稱:食品科學系
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:1994
畢業學年度:82
語文別:中文
論文頁數:165
中文關鍵詞:紫田薯組織培養花青素
外文關鍵詞:Dioscorea alata L. var. purpurea M. Pouchtissue cultureanthocyanin
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本研究探討植物生長調節劑對於紫田薯 (Dioscorea alata L. var.
purpurea M. Pouch) 各培植體產生癒合組織的效果,以薯塊為培植體培
培養時紫紅色癒合組織的誘發程度較高。本試驗範圍內以 MS 培養基中添
加 10 μM 2,4-D 及 0.1 μM kinetin 誘發較多之紫紅色癒合組織。以
部分複因子試驗探討培養基條件對於紫田薯薯塊誘發紫紅色癒合組織之影
響。結果證實,在此試驗範圍內,活性碳、容器種類、蔗糖、氮源、磷酸
鹽、2,4-D 與 kinetin 均顯著影響紫紅色癒合組織之誘發。據此結果,
以反應曲面法尋求誘發紫紅色癒合組織之最適培養基成分。2,4-D濃度及
蔗糖濃度分別為極顯著與顯著的影響紫紅色癒合組織之誘發。以 MF-1 為
培養基以不同光照下培養紫田薯薯塊,結果以太陽燈管照射培養下的紫紅
色癒合組織之誘發程度最高。培植體的最適培養溫度為 23.6 ℃。薯塊與
紫紅色癒合組織所含花青素於不同酸鹼度下之可見光吸收光譜變化情形類
似。隨著 pH 的提升,吸收峰由一個改變至兩個。其顏色變化為:紅色→
紫色→藍色,因此,初步判定紫紅色癒合組織所含的色素為花青素。紫紅
色癒合組織所萃取之花青素於 95 ℃ 加熱 60 分鐘後,仍有 91.82 %
的花青素殘留率。以 100 ml 之 0.1 % 鹽酸甲醇萃取 1 鮮重之紫紅色
癒合組織花青素的吸光值為 0.148,此約為薯塊的 2.11 倍。因此,以紫
田薯組織培養生產花青素的可行性很高。
Effects of plant growth regulators on initiation of purple
calli from explants of Dioscorea alata L. var. purpurea M.
Pouch were studied. Results showed that tuber explants
initiated more purple calli than others. The addition of 10 μM
2,4-D and 0.1μM kinetin to the MS medium induced more purple
calli than that of other combinations. The fractional factorial
experiments were used to investi- gate the effects of cultural
conditions on the initiation of purple calli from the explants
of yams. Results proved that ranges over the tested, charaoal,
type of container, sucrose, nitrogen, phosphate, 2,4-D and
kinetin significantly affected the initiation of calli. From
the results, a response surface methodology was used to search
for the optimum composition of media. The 2,4-D affected very
significantly and sucrose affected significantly the initiation
of purple calli. The tuber explants of yams were cultured on
MF-1 basal medium under different lightings. Results showed
that the degree of purple callusing under the sun light was the
highest. The optimum temperature for culturing explants was
23.6 ℃. The absorption spectrum of pigments from tubers and
purple calli was similar to each other at various pH''s. The
number of absorption peaks changed from one to two with the
increase of pH. The change of color was from red to violet to
blue. Consequently, the pigments of purple calli were
tentatively identified as anthocyanins. The retention of
anthocyanins extracted from purple calli was 91.8 % after 60
minutes at 95 ℃. The O. D. value was 0.148 for 100 ml 0.1 %
HCl-CH3COH extracted from 1 g fresh purple calli. It was 2.11
times to that of tubers. Thus, it is possible to produce
anthocyanins by the tissue culture from yams.
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