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研究生:郭啟中
研究生(外文):Chi-Chung Kuo
論文名稱:不同頻率之電針對於脊髓5-羥色胺及類鴉片接受器之作用機轉研究
論文名稱(外文):Studies of Action Mechanisms of Different Frequencies of Electroacupuncture on Spinal Serotonergic and Opioid Receptors
指導教授:蔡輝彥蔡輝彥引用關係
指導教授(外文):Huei-Yann Tsai
學位類別:碩士
校院名稱:中國醫藥學院
系所名稱:中國醫學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:1995
畢業學年度:83
語文別:中文
論文頁數:67
中文關鍵詞:電針鎮痛脊髓5-羥色胺接受器類鴉片接受器
外文關鍵詞:Electroacupuncture analgesiaSpinal cordSerotonegic receptorsOpioid receptors
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類鴉片物質 (opiate-like substance, OLS) 及 5-羥色胺 (5-HT,serotonin) 媒介針刺之鎮痛作用,已見於發表之文獻,且不同亞型之類鴉片接受器 (opioid receptor) 可能媒介不同頻率電針之鎮痛作用;至於不同亞型之 5-羥色胺接受器 (5-HT receptor) 在不同頻率電針鎮痛作用中扮演何種角色,尚未有進一步的報導。故本研究在探討脊髓中,不同亞型之類鴉片接受器及 5-羥色胺接受器在電針鎮痛之作用機轉。由大鼠髓鞘內插管給藥並以足三里為針刺穴位。在電針鎮痛預試實驗中,於大鼠甩尾試驗結果顯示,1 mA 電針顯現較明顯鎮痛作用,而在3mA 電針於重覆刺激後, 其鎮痛作用減低。故本研究選用電流量 1mA, 以不同頻率 (2Hz, 10Hz, 100Hz) 電針刺激,探討不同接受器作用藥物對不同頻率電針鎮痛之影響。μ-及δ-類鴉片接受器拮抗劑naloxone 及 naltrindole 髓鞘內給藥均可拮抗低頻 2Hz,10 Hz 與高頻100Hz 之電針鎮痛作用;但前者對於低頻的敏感性,大於高頻;而後者對於高頻之敏感性大於低頻。5-羥色胺神經毒素5,7-dihydroxytryptamine (5,7-DHT) 可以阻斷不同頻率電針之鎮痛作用。 5-HT1A 接受器拮抗劑 pindobind-5-HT1A (0.5、5μg)可減低不同頻率之電針鎮痛, 5-HT1A 接受器致效劑 8-OH-DPAT 0.5μg可拮抗高頻電針之鎮痛作用, 但 1μg 及 2μg 可加強低頻電針鎮痛作用。5-HT2/1C 接受器拮抗劑 ketanserin (0.5 μ g) 減弱低頻電針鎮痛?5-HT2/1C 接受器拮抗劑 ketanserin (0.5μg) 減弱低頻電針鎮痛?A5-HT2/1C 接受器致效劑 DOI (10μg) 並未影響電針鎮痛作用。 5-HT3接受器拮抗劑 LY-278584 (0.5、5μg) 可明顯減弱高頻電針鎮痛,5 μg 則對低頻之電針鎮痛亦有減弱作用;5-HT3接受器致效劑 2-methyl-5-HT (50 μg) 則可加強電針鎮痛作用。
由以上結果顯示,在脊髓中μ-及δ-類鴉片接受器均參與電針之鎮痛作用,而μ-類鴉片接受器對低頻電針作用較敏感;而δ-類鴉片接受器則對高頻電針作用較敏感。5-羥色胺接受器 5-HT1A、5-HT2/1C 及 5-HT3 顯現均與電針之鎮痛作用有關,至於主要是影響脊髓中突觸前或突觸後的 5-HT1A、5-HT2/1C及 5-HT3 接受器,則有待進一步之探討。
The possible analgesic mechanisms of electroacupuncture (EAc) in different frequencies were studied by using modified intrathecal catheterization method.
The efficacy of electraoacupuncture analgesia (EAA) in low current (1 mA) are more potent than in high current (2, 3 mA). In addition, high current of EAc stimulation repeatedly may reduce the analgesic effect. Thus, the electric current of 1 mA EAc was chosen to study the mechanism of EAc (2,10 and 100 Hz). The present study was designed to access which subtype of opioid and serotonergic receptors were involved in different frequencies of EAA by the tail-flick test in rats after EAc stimulation at Zu- San-Li. Opioid and serotonergic receptor ligands were administered intrathecally. The results showed that 0.05μg naloxone and naltrindole blocked both low and high frequencies of EAA. The former was more susceptible in low frequency and the latter was susceptible in high frequency of EAc. The effects of EAA in three frequencies were attenuated by pretreatement with 100 μg 5, 7-DHT, implying the serotonergic neuron may participate in the EAA. Furthermore, 0.5,5μg pindobind-5-HT1A could reverse low and high frequencies of EAA; 0.5μg 8-OH-DPAT inhibited the high frequency of EAA but facilitated the low frequency of EAA at doses of 1 and 2μg. Low frequency of EAA was attenuated by 0.5μg ketanserin; 10μg DOI didn''t affect the EAA. 0.5μg LY-278584 attenuated the high frequency of EAA and inhibited the EAA at dose of 5μg. In addition, 50μg 2-methyl-5-HT could enhance the EAA.
These data suggest that the μ-, δ-opioid receptors and 5-HT1A, 5-HT2, 5-HT3 receptors in rat spinal cord are involved in the EAA. The μ- and δ-opioid receptors may play a role mainly in low and high frequency of EAc. However, the exact relationships between EAc and 5-HT subtypes on spinal pre- or post-synapse need
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