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研究生:王之仰
研究生(外文):Wang Chi Young
論文名稱:拓樸異構酵素與假性狂犬病毒複製及基因表現的關係
論文名稱(外文):The roles of topoisomerases in the replication and expression of pseudorrabies virus
指導教授:王孟亮
指導教授(外文):Dr.Wong Min Liang
學位類別:碩士
校院名稱:國立中興大學
系所名稱:獸醫學系
學門:獸醫學門
學類:獸醫學類
論文種類:學術論文
論文出版年:1995
畢業學年度:83
語文別:中文
論文頁數:78
中文關鍵詞:拓樸異構酵素假性狂犬病毒
外文關鍵詞:topoisomerasepseudorabies virus
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假性狂犬病為一種重要的豬的傳染病。假性狂犬病毒為此病的病原,其基
因体為線性雙股DNA其大小約為145kbp,病毒粒子的大小約 150-180nm,
其內部核心由DNA與蛋白質結合而成,包覆核心的蛋白殼由162個
capsomers 組成,為正二十面体結構(icosah- edron),最外圍封套是醣
蛋白與酯蛋白組成,介於封套與蛋白殼之間的不定形結構稱之為
Tegument.其功能仍不清楚。假性狂犬病毒DNA可分為Us(Unique short
region),Ul(Unique long region),及包夾住Us的兩個IR(Inverted
repeat),和(Terminal repeat) DNA複製的機制為rolling-circle模式,
分別由位於Ul和Us上的兩個複製起點開始,病毒本身的DNA合成酵素(DNA
polymerase)及一些輔助因子如DNA binding protein一起擔任合成的工作
。本計畫探討假性狂犬病毒DNA複製及基因表達與拓樸異構酵素的關係,
拓樸異構酵素廣泛存在原核細胞及真核細胞中,控制DNA的拓樸結構構也
影響基因的表達。由理論推測與實驗數據得知,拓樸異構酵素對於基因體
為封閉環狀DNA的病毒(如SV40)之複製十分重要。近來的實驗報告也支持
拓樸異構酵素對線性雙股DNA的病毒的複製與基因表現非常重要。在本研
究中分別利用第一型拓樸異構酵素抑制劑camptothecin和第二型拓樸異構
酵素抑制劑VP-16及 ellipt- icine,發現拓樸異構酵素與假性狂犬病毒
的繁殖有關,更進一步証明假性狂犬病毒DNA的複製需要第一型拓樸異構
酵素參與,我們的結果也顯示在病毒基因表現上的轉錄部分都受第一和第
二型拓樸異構酵素之影響。

Pseudorabies disease is an important infectious disease of
swine, and pseudorabies virus is the causative agent. The
pseudorabies virus genome is a linear double-stranded
DNA(about 145kbp).The icosahedral capsid approximately
150-180nm in diameter contains 162 capsomeres ,
sometimes asymmetric material surrounding the capsid
designated as the tegument, and an envelope containing viral
glycoprotein spikes on its surface.The pseudorabies virus
genome consists of two covalently linked components ,
designated as unique L(long) and unique S(short), which
were bracketed by inverted repeat and terminal repeat.The
viral DNA is synthesized by a virus-encoded DNA
polymerase and a rolling circle replicating mechanism
has been proposed. Our research goal is to elucidate the
roles of topoisomerases in the replication and expression of
pseudorabies virus. Both type I and II DNA topoisomerases
have been found in prokaryotes and eukaryotes. They have
important roles in cellular DNA replication and
transcription. In animal viruses, topoisomerases also involve
in the life cycle of viruses containing closed circular DNA(
such as SV 40 virus) as well as viruses possessing
double- stranded linear DNA(such as adenovirus and
herpesviruses). Our results reveal that type I
topoisomerase inhibitor (camptothecin) and type II
topoisomerase inhibitors (VP-16 and ellipticine) could
inhibit the multiplication of pseudorabies virus. The type I
topoisomerase activity is essential for the DNA
synthesis of pseudorabies virus.Our findings also suggest
that both type I and type II topoisomerases are required for
the gene expression of the pseudorabies virus genome.

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