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研究生:胡志政
研究生(外文):Chih-Cheng Hu
論文名稱:荷蘭種公牛遺傳標記的分析與探討
論文名稱(外文):Investigation of Genetic Markers in Holstein Dairy Bulls
指導教授:毛嘉洪先生
指導教授(外文):Frank Chiahung Mao
學位類別:碩士
校院名稱:國立中興大學
系所名稱:獸醫學系
學門:獸醫學門
學類:獸醫學類
論文種類:學術論文
論文出版年:1995
畢業學年度:83
語文別:中文
論文頁數:97
中文關鍵詞:隨機增殖聚合酵素連鎖反應限制酵素切割多形圖kappa-酪蛋白
外文關鍵詞:RAPD PCRPCR-RFLPskappa-caseinalpha-lactalbuminDNA fingerprints
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本研究以種公牛為分析對象,對其乳蛋白遺傳標記進行基因型鑑定,比較
基因型與種公牛遺傳能力的相關性,並利用隨機增殖聚合酵素連鎖反應(
RAPD PCR)的方法建立種公牛的DNA指紋圖譜。利用聚合酵素連鎖反應併用
限制酵素切割多形圖(PCR-RFLPs)的方法,共分析43頭種公牛的kappa-酪
蛋白基因型,結果AA型有35頭,AB型有7頭,BB型僅有1頭,B型基因頻率
為10.5%,與種公牛遺傳性能評估值相比,顯示AB型基因的種公牛較BB型
種公牛有較高產乳量,乳蛋白量及乳脂量的遺傳能力預測值。在alpha-乳
白蛋白方面,共分析了45頭種公牛的基因型,AA型結果2頭,AB型有18頭
,BB型有25頭,A型基因頻率為24.4%,與種公牛遺傳性能評估值相較顯示
,AA型與AB型種公牛在產乳量、乳蛋白量與乳脂量的遺傳預測方面,有較
BB型為高的趨勢。在指紋圖譜的建立方面,利用RAPD PCR的方法對種公牛
進行 DNA指紋圖譜的構築。經過140種核酸引子的篩選,得到16組指紋,
共擴增出43個較為穩定的產物。並進行重複試驗與個別試驗,以求圖譜的
穩定性。本研究結果將可供酪農做為選擇種公牛冷凍精液的參考,DNA指
紋圖譜的建立亦有助於乳牛遺傳方面的研究,在乳牛育種工作上均具有相
當的潛力。

The objective of this study is to identify the genotypes of
milk protein genetic markers in dairy bulls. The genotype and
predicted transmitting ability of dairy bulls were compared,
and using random amplified polymorphic DNA polymerase chain
reaction (RAPD PCR) to establish the DNA fingerprints of dairy
bulls. Kappa-casein genotypes of 43 dairy bulls were analyzed
using PCR with restriction fragment length polymorphisms (PCR-
RPLPs), and the genotypes identified were 35 AA, 7 BB, and 1
BB. The B gene frequency was found to be 10.5%. Comparison
between genotypes and transmitting trait evaluation indicated
that bulls with AB type gene have higher predited transmitting
ability for the yield of milk, protein and fat than those with
BB type gene. In the alpha-lactalbumin genotyping of 45 dairy
bulls, the genotypes identified were 2 AA, 18 AB, and 25 BB.
The A gene frequency was found to be 24.4%. Comparison between
genotypes and transmitting trait evaluation indicated that
bulls with AA type and AB type gene have higher predicted
transmitting ability. DNA fingerprints were established by RAPD
PCR. By screening with 140 primers, 16 fingerprints and 43
stable amplified products were obtained. The stability of the
fingerprints was confirmed by repeating the tests for
individual and pooled semen DNA. The results of this study can
serve as a reference for dairymen to select the frozen semens
of dairy bulls. The establishment of DNA fingerprints may also
contribute to the improveement in the selection of dairy cattle.

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