|
本試驗以實驗室篩選出台農67號品種水稻之10天胚特有的29個殖系中之
pRE2，pRE3，pRE4，pRE20及pRE28為材料，進行全長定序及分析。其目的
為：(1)探討這些基因的結構與組成；(2)分析這些基因在胚發育過程表現
的時序性及空間性；(3)比較其它植物及品種間的胚發育過程中，這些基
因的表現情形。核酸定序的結果顯示pRE4，pRE3及pRE2的長度分別為1388
，1215及 1087bp，分別可演譯出383，284及267個胺基酸。pRE4與pRE2
有97% 的相似性，僅5'端約有30bp的相異性。pRE2與pRE28有97%的相似性
，pRE4與pRE28有98%的相似性，pRE20的5'端與pRE4有97%的相似性。分析
蛋白質之特性顯示出pRE2,pRE3及pRE4三個殖系皆為親水性區域極多的蛋
白質。以pRE2，pRE3，pRE4，pRE20四個殖系為探針分別與10天，15天
，20天，25天，成熟胚和葉片之mRNA做北方轉漬雜交，結果顯示這些殖系
的表現，以10天胚最高，15天胚稍弱，之後隨時間遞減，在葉片則幾乎偵
測不到。同樣以此四個殖系做探針與10天，15天，成熟胚和葉片之mRNA做
北方轉漬雜交，顯示出pRE2和pRE3可能為全長之cDNA clones。以ABA處理
水稻幼苗均不會誘導pRE2，pRE3，pRE4，和 pRE20 四個選殖系的表現。
NaCl 處理的水稻幼苗，則在四個殖系均略有誘導現象。北方墨點分析的
結果顯示，此四個選殖系在台中 10 號水稻及玉米之十天胚中，有較高的
表現量。
The objectives of the current research are to sequence and
analyze pRE2,pRE3,pRE4,pRE20 and pRE28 of the 29 embryo
specific genes from 10-day embryos of rice(Oryza sativa L. cv.
Tainung 67) .The main purposes are to study the structure and
composition of these genes,to analyze the temporal and spatial
characteristics during embryogenesis,to investigate the
expression of these genes in other species of plants during
embryogenesis. The result of nucleic acid sequencing indicated
the size of insert fragments of pRE4, pRE3 and pRE2 are 1388,
1215 and 1087 bp, respectively, and the number of deduced amino
acid is 383, 284 and 267. The homology of nucleic acid sequence
of pRE2 and pRE4 is 97% and is just different in 30 bp near 5'
end. The similarity of pRE2 and pRE28 is 97%; pRE4 and pRE28 is
98%; 5' end of pRE20 and pRE4 is 98%. The feature of protein
revealed that pRE2, pRE3 and pRE4 are rich in hydrophilic
regions. Highest expression of pRE2, pRE3, pRE4 and pRE20 was
found in 10-day embryos and declined thereafter. The results of
northern hybridization indicated that the pRE2 and pRE3 could
be full length clones. The expression of pRE2, pRE3, pRE4 and
pRE20 could be induced in rice seedlings by NaCl treatment but
not by ABA treatment.
|