跳到主要內容

臺灣博碩士論文加值系統

(44.211.31.134) 您好!臺灣時間:2024/07/24 17:23
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果 :::

詳目顯示

我願授權國圖
: 
twitterline
研究生:孫德芬
研究生(外文):Suen,Der-Fen
論文名稱:以異源染色體添加系從事菸草的基因定位
論文名稱(外文):Gene mapping in nicotiana sylvestris using alien monosomic addition lines
指導教授:陳其昌陳其昌引用關係
指導教授(外文):Chen,Chi-Chang
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:植物學研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:1995
畢業學年度:83
語文別:中文
論文頁數:44
中文關鍵詞:菸草異源染色體添加系
外文關鍵詞:Nicotiana sylvestrischromosome addition linerestriction fragment length polymorphism
相關次數:
  • 被引用被引用:0
  • 點閱點閱:328
  • 評分評分:
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
以含二套N. plumbaginifolia (2n=20)染色體及一套N. sylvestris (n=12)染色體的異源三倍體體細胞雜種(2n=32)與二倍體
N. plumbaginifolia回交一與二次,得到 93株含二套N. plumba-
ginifolia及一條N. sylvestris染色體的異源單染色體添加植株。根據外形這些植株可分成13組,每組植株的外形各有特徵。以61個探針進行限制片段長度多型性分析,有13個探針在所有的染色體添加植株均偵測不到N. sylvestris的DNA,其餘48個探針(包括rDNA及NR基因)將染色體添加植株分為9個正常的添加系,及4個染色體轉座或丟失的變異型添加系。正常的添加系中有四個添加系的N. sylvestris 染色體已鑑定為 sat-1、sat-2、sat-3及第12條短的中位中節染色體,另5個系的染色體未鑑定出,只歸為N.sylvestris 的A、B及C組染色體。其中有一株在顯微鏡下觀察到染色體丟失。另外有一植株外形特殊,但在染色體觀察與限制片段長度多型性分析並沒有發現變異。此外,在體細胞雜種中也偵測到N. plumbaginifolia的DNA變異。本論文並發現,sat-3染色體的rDNA在二倍體N.sylvestris 中不表現,但在染色體添加植株中則可偵測到其活性。

An allotriploid hybrid (genomes PPS) obtained from proto-plast fusion between haploid Nicotiana plumbaginifolia (P, n=10) and haploid N. sylvestris (S, n=12) was backcrossed to monosomic chromosome addition lines. A total of 93 2n=21 plants, each containing a 2n chromosome complement of N. plumbaginifolia and a single chromosome of N. sylvestris, appeared in the BC and BC ge- nerations. The 2n=21 plants could be classified into 13 groups based on morphological characteristics. The N. sylvestris chromosomes in the 2n=21 plants were determinated by RFLP markers and karyotype analysis. Of the 61 RFLP markers ana- lysed, 13 could not detect N. sylvestris DNA bands in the 2n= 21 plants, whereas the remaining 48 markers (including rRNA and NR genes)identified 9 normal and 4 aberrant lines. The N. sylvestris chromosomes in the normal lines were chromosomes 9(sat-1), 10 (sat-2), 11 (sat-3), 12, one of the A group, one of B group, and three of the C group, respectively. The N. sylvestris chromosomes in the aberrant lines could not be identified with certainty, but presumably they were translo- cated or deleted chromosomes. One morphologically distinct 2n=21 plant showed no chromosomal and DNA changes judged by karyotype and RFLP analysis. The rRNA genes in sat-3 chromo- some do not usually express in diploid N. sylvestris, but are active when added to the genetic background of diploid N. plumbaginifolia.

QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top