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研究生:王俊堯
研究生(外文):Wang, Jun Yao
論文名稱:低溫刺激對於虱目魚和草魚醣類代謝調控之探討
論文名稱(外文):Regulation of carbohydrate metabolism in milk-fish (chanos chanos) and grass carp (ctenopharyngodon idella) under cold shock treatments
指導教授:郭欽明郭欽明引用關係
指導教授(外文):Guo, Qin Ming
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:漁業科學研究所
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:1995
畢業學年度:83
語文別:中文
論文頁數:101
中文關鍵詞:低溫刺激魚類醣類代謝
外文關鍵詞:cold shockfishcarbohydrate metabolism
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虱目魚為狹溫性魚種,僅適於高溫下養殖,每當寒流過境,虱目魚往往因不耐低溫而大量死亡。本篇論文之研究目的係探討在低溫刺激下,比較虱目魚與耐寒之草魚醣類能量代謝之酵素活性調節情形。草魚與虱目魚在低溫壓迫期間,血液滲透壓均較平常為低,然而虱目魚在低溫致死的階段出現大幅下降現象。在血糖濃度方面,虱目魚與草魚在低溫壓迫階段,血液中葡萄糖含量顯著增加,又以虱目魚所增加的幅度為大。虱目魚血液中葡萄糖的來源,主要是經由糖質新生作用產生的,肝糖分解之酵素活性雖然在低溫刺激下有增高的現象,但與血糖濃度之增加無必然結果。虱目魚在15℃低溫壓迫的後期,以及12℃的刺激下,血糖濃度呈現大幅下降的現象,顯示出在低溫壓迫之致死階段,葡萄糖的供應上有所阻礙。而在糖解作用以產生能量的代謝途徑上,經由測定虱目魚及草魚的肝臟與肌肉的LDH, PK, CS和G6PDH之活性,發現草魚在低溫刺激下主要是增強有氧糖解作用以獲得較多的能量,以及五碳糖的代謝途徑以產生NADPH而有利於脂質的生合成。而虱目魚在低溫下這些代謝途徑的增強效果並不顯著,甚至有降低的趨勢,顯示出虱目魚對葡萄糖的利用率並不高,這可能是其無法在低溫下長期存活的原因。另一方面,虱目魚在低溫下血糖濃度增加之幅度很大,表示其在產生葡萄糖的代謝過程上受到相當大的激活。研究發現在12℃低溫培養下的肝組織,在葡糖的釋放量以及糖質新生和肝糖分解之酵素活性,均較25℃下培養為高。Catecholamines對促進葡萄糖釋放肝糖分解的效果極為顯著,其中又以norepinephrine 較epinephrine效果為佳。然而catecholamine對糖質新生的增強效果並不顯著。這些結果顯
示catecholamine在某種程度上參與了調節葡萄糖的產生與釋放。然而在無外界因子參與下,細胞本身單純受到低溫的刺激,也能激發特殊的調節機制。
Milkfish, Chanos chanos, are stenothermal fish, which have
low tolerance to low temperature. To study the cold shock effects on the regulation of carbohydrate metabolism, the eurythermal fish - grass carp, Ctenopharyngodon idella, was also studied to compare energy production and utilization of these two spicies at low temperature.Milkfish under 15℃ cold shock treatment, the blood glucose content increased 5 folds, and both the activities of gluconeogenicc and glycogenolytic enzymes increased. But in 12℃ cold treated milkfish and the late period of 15℃ cold shock, the blood glucose content decreased. It showed the glucose supply were shorted under extreme or prolong cold shock.The activities of PK, G6PDH, LDH, and CS in the liver and muscle of milkfish and grass carp under cold shock were measured. The results indicated that milkfish had enhanced aerobic glycolysis to produce more ATP under cold shock, grass carp favored in aerobic glycolysis and enhanced the pentose phosphate pathway to produce NAPDH for lipid biosynthesis.
In addition, in vitro cultured milkfish liver was used to study how low temperature and catecholamines influence glucose
production.The results showed that 12℃-cultured liver tissue
releasd more glucose and had higher enzyme activities of
glycogenolysis and gluconeogenesis than 25℃-cultured liver
tissue. The effects of norepinephrine on the increae of hepatic
glucose release were significant. Epinephrine reduced the glucose release, but it activated glycogenolysis. Norepinephrine affected the increased of gluconeogenesis more significantly than epinephrine.These suggested that except of stress hormones, liver cells themself could regulate hepatic glucogenolysis and gluconeogenesis.
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