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Phyllostachys pubescens is a bamboo species with
leptomorphrhizomes. It is an economically important bamboo in
Taiwan. Theamplifying genomic DNA segments using RAPD,
microsatellite, andminisatellite sequences as primers and to
investigate the purposeof the present study is to identify
clones (genotypes) bydistribution of clones in Taiwan. One
hundred and seventy-eightsamples of the species, together with
5 samples of Phyllostachyspubescens cv."Tao Kiang" and 4
samples of Phyllostachys pubescensvar. heterocycla were
collected around the island. Genomic DNAfragment were
amplified using 13 RAPD primers, 3 microsatellitepatterns were
used for clone identification. Nine clones wereidentified
form 178 samples of the species. Seven clones wereidentified
from samples of cultivar and the variety. Phyllostachys
pubescens is reproduced mainly by rhizome. Itsgenetic variation
is generally expected to be limited. The mainsource of
variation is resided within sampling sites. Thereis no
significant genetic diversification between sites andbetween
regions. The observed clones in the present study mightbe
introduced from mainland china couple hundred years ago
andmaintained to the present day. However, we can not rule out
thepossibility that some of the clones might be originated
throughtsomatic mutation. The results also showed that the
regions aroundNan-Tou County consisted of all of the 9 clones of
the specieswhile the rest regions generally consisted of only 1
commonclone. This evidence indicated that Nan-Tou region might
be thefirst location of cultivation and later the cultivation
spreadedto other regions.
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