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研究生:吳信頡
研究生(外文):Wu, Shinn-Tye
論文名稱:鹽分逆境對水稻生理代謝與微細構造之影響
論文名稱(外文):Effects of Salt Stress on Metabolism and Ultrastructure of the Rice (Oryza sativa L.)
指導教授:廖松淵
指導教授(外文):Liaw Song-Luan
學位類別:碩士
校院名稱:國立中興大學
系所名稱:植物學系
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:1996
畢業學年度:84
語文別:中文
論文頁數:94
中文關鍵詞:水稻鹽分逆境碳代謝微細構造
外文關鍵詞:riceOryza sativa L.salt stresscarbon metabolismultrastructure
相關次數:
  • 被引用被引用:2
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  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:1
本研究以 75 mM NaCl、150 mM NaCl 處理水耕栽培之水稻,並分別
探討鹽分處理下,水稻葉片與根部組織之生理與微細構造的變化。結果發
現鹽分處理導致供試植株生長受抑制,且氣孔導度與光合成速率也隨鹽分
處理濃度增加而減少。鹽分處理亦會造成呼吸速率的下降,且根部組織交
替途徑的活性因鹽分處理而增加,但其交替途徑活性增高程度隨著水稻品
種耐鹽與否而異,IR34 在鹽分處理第 16 天時,交替途徑已開始下降;
反之 TNS19 在鹽分處理第 16 天時,其交替途徑活性仍高於對照組。水
稻在鹽分處理時,其葉片與根部組織的可溶性糖含量皆隨鹽分處理濃度而
增加,可溶性糖的增加,一可增加滲透調節物質,二可增加呼吸作用的基
質,且發現葉片的可溶性糖主要是蔗糖,而根部組織則以葡萄糖為主。耐
鹽品種水稻(TNS19)葉片與根部組織 SPS 的活性皆隨著鹽分處理濃度增
加而增加;不耐鹽品種(IR34)葉片 SPS 的活性亦增加,但根部組織的
SPS 則呈下降之趨勢。此外,有關蔗糖分解的酵素(SS、NI 及 AI)活性
在鹽處理時均有下降的趨勢,而澱粉的含量亦隨著鹽分處理而減少。由上
述可知,鹽分處理時,植物體內會提高可溶性糖含量,以做為滲透調節物
質與呼吸基質。在游離胺基酸方面,鹽分處理會造成脯胺酸(proline)
的大量累積,以做為滲透調節物質,且根部組織在麩胺酸醯胺(Gln)、
麩胺酸(Glu)、天門冬醯胺(Asn)及天門冬胺酸(Asp)的含量皆有大
幅增加,可能與氮源的再利用及運輸有密切關係。另外,由 SDS-PAGE 發
現,鹽分處理可誘導水稻根部組織合成新的蛋白質。水稻鹽分處理後,微
細構造之變化主要為葉綠體的類囊體膜,由原來平行堆積,逐漸有剝離崩
解的現象,且葉綠體內的澱粉粒也有減少的現象;根部組織在鹽分處理後
,細胞大多只剩大型液泡,甚至只剩下細胞壁。此等微細構造變化程度亦
因供試水稻品種耐鹽與否而異。

The research is using 75mM NaCl and 150mM NaCl to deal with
the rice inhydroponic culture system, and discussing the
physiological change andultrastructure of the rice leaves and
its roots under salt stress. The resultis found that the growth
of the rice is inhibited under salt stress, and thestomatal
conductance and photosynthetic rate will also decrease when
theincreasing concentration of salt stress . Salt stress will
also cause thedecreasing of the respiration rate, and the
activity of alternative pathway ofroots will increase because
of salt stress, but the increasing leavel willdepend on whether
the rice is salt-tolerate or not. On the 16th day of IR34under
salt stress, the alternative pathway has already start to
decrease; onthe opposite, the activity of alternative pathway of
TNS19 under salt stressis still higher than the control one.
When the rice is under salt stress, thesoluble sugar content in
its leaves and roots will increase with the
increasingconcentration of salt stress. The increase of soluble
sugar content can increasecompatible solutes and respiration
materials. And the soluble sugar in leavescan be found mostly
sucrose, and in roots mostly glucose. The salt-toleraterice
(TNS19), the activity of SPS of leaves and roots will increase
with theincreasing concentration of salt stress; the salt-
sensitive rice (IR34), the activity of leaves also increase, but
one of roots tends to decrease. In addition,as for the enzyme (
SS, NI, and AI ) which hydrolyzed sucrose, the activity hasthe
decreasing tendency under salt stress. And starch content will
also decreasess. Thus, under salt stress, the plant will
increase the soluble sugar contentto be used as compatible
solutes and respiration materials. As for free aminoacid, salt
stress will cause a greataccumulation of proline, which can be
usedas compatible solutes, and the roots have a great increasing
on the content ofglutamine, glutamate, asparigine, and asparate.
The situation of which maybehave a close relation with the
reutilization and transport of nitrogen.Moreover, according to
SDS-PAGE, it can be found that salt stress will inducethe new
synthetic protein.Under salt stress, the main change of
ultrastructureis that the thylakoid of chloloplast is gradual
breakdown from the parallelstack, and the starch in chloroplast
also have be hydrolyzed; the root cellsmostly become some big
vacuole, or even some cell wall. The change level ofthe
ultrastructure also will be different along with whether the
rice issalt-tolerate or not.

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