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研究生:蔣青蓉
研究生(外文):Chiang, Ching-Jung
論文名稱:維生素B6協同胰島素治療糖尿病大白鼠的血小板凝集及血管變化研究
論文名稱(外文):Study of vitamin B6 in conjunction with insulin treatment of STZ-induced diabetic rats on the platelet aggregation and the vascular and endothelium
指導教授:張素瓊張素瓊引用關係
指導教授(外文):Chang Sue-Joan
學位類別:碩士
校院名稱:國立成功大學
系所名稱:生物學系
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:1996
畢業學年度:84
語文別:中文
論文頁數:86
中文關鍵詞:糖尿病維生素B6血小板凝集
外文關鍵詞:DiabetesVitamin B6Platelet aggregation
相關次數:
  • 被引用被引用:2
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血管疾病併發症是糖尿病死亡的主要原因。糖尿病的血管疾病併發症
主要是動脈硬化所造成的。血管內皮細胞損傷被認為會引發動脈硬化症。
致血栓因子,包括血小板過度活化或血小板過度凝集,均會促進動脈硬化
的過程。維生素B6具有保護血管內皮細胞、減少內皮細胞受活化血小板損
傷的作用、抑制血小板凝集與血液凝固、抑制血小板生成前列凝素(
Thromboxane A2,TxA2)及促進血管內皮細胞生成環前列腺素的作用(
Prostaglandin I2,PGI2)、以及影響血管內皮細胞形態上的改變。故維生
素B6可能具有抗血栓、抗動脈硬化的作用。 本研究主要目的在探討維
生素B6是否可減緩胰島素治療的糖尿病血管病變。以Streptozotocin(
STZ)誘導糖尿病大白鼠,分別施以胰島素、維生素B6或胰島素與維生素B6
同時治療。以腺 磷雙磷酸(ADP,10μM)或凝血(Thrombin,0.05U/mL)
當致凝物,在血小板凝集儀下測定血小板凝集程度。以放射性花生四烯
酸(14C-Arachidonic Acid,14C-AA)為前驅物,利用放射性薄層層析(
Radiochro-matography Scanning)及自動放射照像(Autoradiography)分
析血小板生成前列凝素(14C-Thromboxane B2,14C-TxB2)的情形,並用放
射線閃爍計數器(Scintillation Counter)測量14C-TxB2生成量。同時利
用掃描式電子顯微鏡觀察血管壁組織學上的變化。 實驗結果發現,胰
島素與維生素B6同時治療的實驗組,血糖值低於胰島素或維生素B6單獨治
療的兩組。同時血小板凝集程度(以ADP,Thrombin當致凝物)及血小板生成
前列凝素程度亦顯著降低。血糖值與血小板凝集程度及血小板生成前列凝
素呈正相關。掃描式電子顯微鏡觀察到較少的血小板與血球附著於血管壁
。以上結果皆顯示,維生素B6可減緩胰島素治療糖尿病大白鼠之病情與血
管併發症的誘因,對血糖控制、防止血小板凝集、減少前列凝素生成、避
免血球附著血管壁皆比胰島素單獨治療的效果好。因此,維生素B6協同胰
島素治療糖尿病,對預防或減緩糖尿病晚期血管併發症的發生與惡化應有
其正面的影響。
The vascular complication has been considered to be one of
the major risk factors of mortality in diabetes mellitus. The
principal cause of this vascular disease is atherosclerosis.
Endothelial injury has been suggested as a potential initiating
event in the development of atherosclerosis. It has also been
postulated that thrombogenic factors, such as platelet
hyperactivity or platelet hyperaggregation, may be an early
pathological process in atherosclerosis. Vitamin B6 has been
found to contribute to protect the vascular endothelial injury
from platelet activation; inhibit the platelet aggregation and
blood clotting; inhibit thromboxane A2 production of platelet;
increase prostaglandin I2 production of the vascular endothelial
cells and affect the morphology of vascular endothelial cells.
It is possible that vitamin B6 may exert certain antithrombotic
and antiatherogenic effects. The purpose of this study was
to investigate whether vitamin B6 alleviates the vascular
complications of insulin-treated streptozotocin (STZ) -induced
diabetic rats. Diabetic animals were treated with or without
vitamin B6 and / or insulin. Platelet aggregation induced by
ADP (10μM) or Thrombin (0.05 U/mL) was measured in plasma rich
platelet of normal and diabetic animals. 14C-thromboxane B2
(14C-TxB2) production of platelet , using 14C-arachidonic acid (
14C-AA) as a precursor, was assayed by means of
radiochromatography scanning and autoradiography. 14C-TxB2 was
quantified by scintillation counter. The results showed that
vitamin B6 in conjunction with insulin treatment revealed lower
blood glucose than either vitamin B6 or insulin treatment alone.
Similarly, platelet aggregation induced by ADP or Thrombin and
TxB2 production of diabetes with vitamin B6 and insulin
treatment were significantly decreased. Blood glucose
concentrations were positively correlated to the platelet
aggregation or thromboxane B2 production. Scanning electric
microscopy showed a decreased platelets and blood cells adhesion
to the vascular wall. These data indicated that vitamin B6 in
conjunction with insulin treatment seemed to be better than
vitamin B6 or insulin treatment alone in controlling blood
glucose, inhibiting platelet aggregation, decreasing TxB2
production and preventing platelet and blood cells adhesion to
the vascular wall. In summary, vitamin B6 in conjunction with
insulin treatment of diabetes mellitus might provide a possible
role in preventing or reducing the progress of the vascular
complications in later stage of diabetes mellitus.
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