臺灣博碩士論文加值系統

細胞膜上之磷脂質經磷脂 A2 作用產生花生四烯酸，再經由三條不同的
路徑產生不同的 代謝物，包括前列腺素、前列凝素及白三烯素等，這些
產物都具有很強的生理功能，其中白三烯素與過敏及發炎反應有密切的關
係。

氣喘是由於呼吸道受過敏原刺激，產生了發炎反應，造成呼吸道阻礙及平
滑肌不正常收縮的疾病。在許多研究中發現，引起此發炎反應的主要細胞
為肥大細胞及單核球－巨噬細胞。當這些細胞經過敏原刺激後，會釋放不
同的媒介物引起氣喘反應，包括白三烯素。藉由研究肥大細胞及單核球－
巨噬細胞中白三烯素代謝之調控機制，可對治療氣喘疾病提供有用的資訊
。 在本研究中，我們以肥大細胞株 P815 及單核球－巨噬細胞株
P388D1 為研究對象，測試三種市售具有抗氣喘療效的中藥材五味子、陳
皮、杏仁對白三烯素生成的影響，並研究 PKC、Ca2+ 對其之調控情形。
實驗設計上，除三種中藥材外，並使用 PKC 活化劑、抑制劑及Ca2+ 離子
載體 A23187 進行花生四烯酸代謝研究。每次實驗平均使用 107 個細胞
加入緩衝液中與 0.1 mCi 花生四烯酸及待測藥物共同培養 2 小時，代謝
產物經萃取，以HPLC 分離後分析白三烯素的消長情形。 實驗結果顯
示，三種中藥材對肥大細胞株 P815 及單核球－巨噬細胞株 P388D1 白三
烯素 C4/D4 生成皆具有抑制的效果，其中以五味子最佳，其次為杏仁與
陳皮。在調控方面，P815 細胞之白三烯素 C4/D4 生成受 PKC 的正向調
控，但不受 Ca2+ 所影響。而 P388D1 細胞之白三烯素 C4/D4 生成則完
全不受 PKC 及 Ca2+ 所調控。

The phospholipids of cell membrane can be metabolized to form
arachidonic acid by phospholipase A2. Arachidonic acid then
produces different metabolites, including prostaglandins,
thromboxanes and leukotrienes by three distinct pathways. These
products have potent biological functions, particularly
leukotrienes, related with allergy and inflammation. Asthma
is a disease that bronchia are stimulated by allergens resulting
in inflammatory reactions with contraction of brochi and
dyspnea. In some studies, it has been found that the major cells
involved in inflammatory reactions are mast cell and monocyte-
macrophage. When these cells are challenged by allergens, they
can release different mediators such as leukotrienes to induce
asthma. If we understand the regulatory mechanisms of
leukotriene production in mast cell and monocyte-macrophages, we
may be able to control the asthma. In this study, we
investigated the effects of three different Chinese herbs,
Schisandra chinensis Baill, Citrus tangerina Hort. et Tanaka and
Prunus armeniaca L., on the leukotriene production in mouse
mastocytoma cell line P815 and mouse monocyte-macrophage cell
line P388D1. Furthermore, regulation of leukotriene production
by PKC and Ca2+ in these cell lines was also studied. These
Chinese herbs, PKC activators and inhibitors, and Ca2+ ionophore
A23187 were used to study the effects on arachidonic acid
metabolism. Test cells were resuspended in buffer (pH = 7.4) and
incubated with 0.1 mCi 14C-arachidonic acid and above agents for
2 hr at 37℃. The products of arachidonic acid metabolism were
extracted and then identified by HPLC. Results show that
these three Chinese herbs have inhibitory effects on the
production of LTC4/D4 in P815 and P388D1 cells. The inhibitory
effects of Schisandra chinensis Baill is shown to be the
highest, and Citrus tangerina Hort. et Tanaka and Prunus
armeniaca L. were the next. In P815 cells, it has been found
that activation of PKC contains position effect on the
regulation of leukotriene production. However, high
concentration of Ca2+ did not affect leukotriene production. In
P388D1 cells, both PKC and Ca2+ show no effect on leukotriene
production.