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There were two major stages in this study. In the first stage the characteristics of both Candida tropicalis and yeast fusant were studied, the difference between them examined. The results from this stage were then used as the controlling parameters in next stage, in which the feasibility of using yeast fusant in treating industrial phenolic wastewater was investigated. The results show that, at the optimal temperature and pH for degrading phemol with C. tropicalis was 32℃ and 6∼7, respectively. Similarly, for degarding phenol with yeast fusant, the optimal temperature and pH were, respectively, 30∼32℃, and 6∼7. Under these optimal conditions, the highest concentrations of phenol degraded by C.tropicalis and by yeast fusant were 2,787 mg/L and 3,280 mg/L, respectively. If the safety factor is not considered, to meet the 1993 standard of effluent concentration in petrochemical industry (R.O.C. EPA, COD=250 mg/ L, phenol=2 mg/L), the phenol volumetric loading in this biological treatment system should be lower than 1.72 kg/m3-day, and the COD volumetric loading lower than 6.54kg/m3-day.When the COD volumetric loading was raised to 7.45kg/m3-day, the effluent COD concentration was 323.9mg/L. However, at the same time, the effluent phenol concentration remained lower than 1mg/L. This implies that some non-phenol organics could not be easily degarded, causing the effluent COD concentration to be higher than the normal level.
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