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Shoot tip culture of the Wutai persimmon (Diospyros japonica
Sieb. et Zucc.)was studied, optimal micropropagation conditions
for culture establishment, sh-oot proliferation, shoot rooting
and acclimatization were investigated. Shoottips were harvested
about 5cm in length from actively field grown trees and sh-oot
apices were excised 0.2- 0.3 mm for initial culture. The best
grown were achieved when shoot apices cultured on solid medium
with 1/2 strength of Mura-shige and Skoog (MS)containing zeatin
1 mg/l,sucrose 30 g/l and agar 7 g/l andincubated in darkness
for 1 days, then incubated in lightness for 29 days. Th-ese
explants were transfered to the same component solid medium for
further gr-owth. Multiple shoots were induced by MS medium
cotaining sucrose 30 g/l and agar 7g/l plus BA 5 mg/l, 10 mg/l
or 2ip 5 mg/l,10 mg/l or zeatin 1 mg/l for 40 days.By using
double-phase medium culture, the multiplication rate of expla-nt
could be increased. For rooting, the shoots grew above 20mm in
height, we-re placed on 1/2 MS medium with IBA 10 mg/l, sucrose
30 g/l and agar 6 g/l andincubated in darknessfor 7 days, then
transplanted to an auxin-free 1/2 MS me-dium for 23 days. Rooted
plantlets were gradually acclimatized during two wee-ks, and
planted inplastic baskets filled with peatmoss and perlite. The
plant-lets became healthy and developed vigorous roots after
five months.
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