臺灣博碩士論文加值系統

磁振造影使用於醫學臨床診斷上，具有較其他影像學診斷工具更好的病變
區域解剖構造呈現。因此，目前已成為對中樞神經系統疾病最重要的影像
學診斷工具。本研究主要是開發適當的貓急性缺血性腦中風動物模式。因
為，近年來急性缺血性腦中風的患者有逐年增加的趨勢，而國內迄今沒有
相關的腦中風動物模式，以進行磁振造影影像學的研究。同時，使用兩種
臨床醫學上的磁振造影射頻脈衝T2-weighted imaging (T2WI)及
Diffusion-weighted imaging (DWI)，配合血清肌氨酸激電泳分析，檢
測此模式是否可行。再以此動物模式對於Magnetization contrast
imaging (MTC)、T2WI及DWI此三種不同的磁振造影射頻脈衝，對急性缺血
性腦中風診斷成效進行比較。主要結果如下：一、貓長時間麻醉目前使用
Thiamylal進行實驗貓的維持麻醉。每隔1小時補強的情況下，維持實驗貓
穩定的外科麻醉期，超過26小時的總麻醉時間。並且本藥物對於實驗動物
的肝、腎功能的毒性傷害亦相當低。二、貓急性缺血性腦中風的實驗手術
經由磁振造影及血清肌氨酸激電泳分析，已成功發展出穩定且可重複性
高，永久性的貓急性缺血性腦中風動物實驗模式。三、磁振造影射頻脈衝
及診斷效能的比較發現T2WI在長時間(>12小時)，對於缺血病灶的診斷能
力較DWI及MTC良好，而DWI及MTC在缺血發生後6小時的診斷成效相同。T2
WI的影像與組織病理切片中的病灶區域完全吻合，而DWI及MTC則不同。

Magnetic resonance imaging (MRI) offers better soft tissue
contrast than most other diagnostic imaging techniques. For this
reason, it is fast becoming one of the most important clinical
imaging techniques for the diagnosis of central nervous system
in recent years. An appropriate animal model is essential for
the study of etiology of all diseases. Further more, the brain
ischemia and acute stroke is has become the leading cause of
death in Taiwan recently. Yet, there is so far no appropriate
stroke animal model developed for MRI studies. To fill this
void, the strive for the development of an appropriate animal
model for cat acute ischemic stroke and an appropriate
anesthetization method for MRI examination becomes our main
goal. Our method can be divided into two stages. First, serum
creatine kinase (CK) electrophoresis and two MRI diagnosis pulse
sequences 'T2 - weighted imaging (T2WI)' and 'Diffusion -
weighted imaging (DWI)' are used for the feasibility inspection.
Secondly, the efficacy of three MRI methods: Magnetization
transfer contrast (MTC) along with two mentioned above in these
use as a diagnostic modality on stoke animal model are
evaluated. The main results of this study can be stated as
following: 1. Long term anesthesia of cat:In this study,
'Thiamylal' is found to be a good anesthesia for cat, as it
provides a stable surgery period (26 hour). The other good
feature of thiamylal is its lower toxicity for liver and kidney
of the laboratory animals.2. Experimental surgery of cat acute
ischemic stroke:Highly stable and feasible animal model can be
successfully developed by used of CK electrophoresis analysis
and MRI diagnosis method.3. The Comparisom of three MRI methods:
In indentifyng long lesion term(>12 hours), T2WI appears to be,
based on our results a better technique than DWI and MTC.
However, the DWI and MTC techniques seem to be superior in
providing earlier detection of lesions again based on our
preliminary findings. The gross finding result devotailed the T2
WI result. But the DWI and MTC results are not devotailed the
gross finding result.