|
Spores were obtained from the greenhouse at TaiTung
DAIS.The methods of specimen preparation and observation on SEM
were divided four treatments.Treatment A:The spores only air
dried two weeks and storage at 6 months.Treatment B:Spores
including sporangia were put in the tube.About 10 c.c ofroom-
temperature glacial acetic acid was added to the tube and kept
in the boiling water bath for about 1 minute.Treatment C:Spores
were placed in 2.5%Glutaraldehyde+0.05Mphosphate buffer(pH7.2),
Samples were rinsed in 95% ethanol at 5 times and dehydrated in
95% ethanol.Treatment D:Fresh spores only.The best result is
treatment D,fresh spores were mounted on standard stub and
sputter coated with gold palladium for 1 minute. This
project had been collect 29 species of indigenous Pteridophytes
in Particular those belong to Thelypteridaceae and
Polypodiaceae,from east Taiwan and establish a plantation tor
the conservation of all the species collected.Fresh spores had
been sampled directly from the plantation and examined withSEM.
Based on the fine structure of extine ornamentation,aconcise key
to the inidgenous Pteridophytes of Taiwan had been established.
Tree fern debris is the best germination for leatherleaf fern
propagation by using spore.The best tion for leatherleaf fern
spore sowing is autumn in Taitung district because high summer
temperature can affect the prothallium growth and cause it
browning even dying.Leatherleaf fern,a sciophyte,requires shade
for commercial production.Desired shade level,according to the
ferneries located,must be adjusted properly.The results of
cultivation trial in Taitung district show that 80%shade level
had superior cut leaf yield,leaf length,leaf weight to 40% shade
level.THere have significant difference between them.Low shade
level may cause the leaf color yellow,the short cut leaf,and no
economical value.
|