臺灣博碩士論文加值系統

培養在不同鐵濃度（分別為：不添加、添加10-8M、10-7M、10-6M、10-5M
、10-4M及10-3M檸檬酸鐵）下，兩種綠藻（Tetraselmis sp.、
Dunaliella sp.）、兩種矽藻(Skeletonema costatum、Bellerochea
horologicalis）及一種藍綠藻(Synechococcus sp.）的生長速率、單位
細胞內葉綠素a 及類胡蘿蔔素含量，除藍綠藻的生長速率是隨鐵濃度增加
而減少外，皆隨鐵濃度增加而上升。另將先前培養在加鐵(10-4M）及未加
鐵兩種培養液中的Dunaliella sp.、S. costatum及Synechococcus sp.，
分別移至加鐵（10-4M）及不加鐵的培養液中，所得短時間營養鹽（鐵、
磷酸鹽及硝酸鹽）的吸收速率大都以持續培養在加鐵培養液中最高，而持
續培養在缺鐵中的為最低。相同處理下，S. costatum及Synechococcus
sp. 所得光合作用的結果亦與營養鹽吸收有相同的情形。以 SDS 膠質電
泳分析培養在不同鐵濃度（不加鐵、10-7M 及10-4M 檸檬酸鐵）下，五種
藻細胞的蛋白質含量及組成， Tetraselmis sp. 在分子量接近25 kDa，
Dunaliella sp. 在 23 kDa 左右，Synechococcus sp. 於 26 kDa 左右
的蛋白質是在加鐵的處理中含量較高。 Tetraselmis sp. 在 22 kDa 左
右的蛋白質、Dunaliella sp. 在 33 kDa 左右的兩個蛋白質及接近 40
kDa 的蛋白質、Synechococcus sp.在 95 kDa 左右蛋白質的含量，則在
不加鐵或鐵濃度低（10-7M）時較高，矽藻的蛋白質變化較不明顯，主要
一些低分子量的蛋白質有些微的變化。五種藻細胞內游離胺基酸的含量及
百分比皆以 glutamate、glutamine及 alanine 較高。不同種類間另含有
不同的主要游離胺基酸，如 Tetraselmis sp.，含有較高的 arginine，
S. costatum 及 Dunaliella sp. 的浮游植物則含較多的 asparate，後
者 alanine 的量亦多。不同藻細胞內游離胺基酸的含量，隨培養液中鐵
濃度的不同而有不同的變化，如 Tetraselmis sp.的 glutamate、
alanine；S. costatum 的 asparate、alanine；B. horologicalis 的
asparate、aspargine、arginine 及 Synechococcus sp.的 asparate、
alanine 是隨鐵濃度上升而增加；Tetraselmis sp.及 Dunaliella sp.
的arginine，S. costatum 的 glutamate、tyrosine；B. horologicalis
的 glutamine、tyrosine 及 Synechococcus sp. 的 glutamine 則隨鐵
濃度增加而減少。細胞外游離胺基酸在五種藻類中則以 serine、glycine
、alanine 及 glutamate 含量最高，其中，Tetraselmis sp.的 serine
、tyrosine，S. costatum 及 B. horologicalis 的 serine、glutamate
，Synechococcus sp.的 glutamate 含量是隨鐵添濃度增加而上升；
Dunaliella sp.的 serine、glycine、tyrosine，S. costatum 及 B.
horologicalis 的glutamine、glutamate 及 Synechococcus sp. 的
serine 則隨鐵濃度增加而下降。

The growth rate, pigment content, nutrient uptake and chemical
composition of five marine phytoplankters ( Chlorophyceae:
Tetraselmis sp. and Dunaliella sp.; Bacillariophyceae:
Skeletonema costatum and Bellerochea horologicalis;
Cyanophyceae: Synechococcus sp.) in the cultures with or without
addition of ferric citrate ( 0-10-3M) were determined. The
results indicated that growth rate, chlorophyll a and
carotenoids increased with increasing iron addition, exclusive
of the lower growth rate of Synechococcus sp. with increasing
external iron. The uptake rate for nutrients (iron, phosphate
and nitrate) in Dunaliella sp., S. costatum and Synechococcus
sp. provided with iron (10-4M) was higher than that without iron
addition. Similar result was also found in photosynthetic rates
of S. costatum and Synechococcus sp. By using SDS gel
electrophoresis, a marked increase of proteins near 25 KDa, 23
KDa and 26 KDa molecules was observed in Tetraselmis sp.,
Dunaliella sp. and Synechococcus sp., respectively, if Fe was
added to the culture medium. However, the proteins near22 KDa,
33 KDa,40KDa and 26KDa molecules appeared to be increased in
Tetraselmis sp., Dunaliella sp. and Synechococcus sp.,
respectively, grown with less Fe (10-7M) or without Fe addition.
With diatom S. costatum and B. horologicalis, the variation of
protein composition with Fe treatment was less apparent. Of the
intracellular free amino acids determined, glutamate, glutamine
and alanine were most abundant in the algae studied. In
addition, arginine and asparate were also present in higher
levels in Tetraselmis sp. and S. costatum, respectively.
However, glutamate and alanine in Tetraselmis sp.,asparate and
alanine in S. costatum, asparate, asparagine and arginine in B.
horologicalis and asparate and alanine in Synechococcus sp.
increased markedly with increasing Fe in the medium. In
contrast,arginine in Tetraselmis sp. and Dunaliella sp.,
glutamate and tyrosine in S. costatum, glutamine and tyrosine in
B. horologicalis and glutamine in Synechococcus sp. decreased
with increasing external iron. Meanwhile, certain free amino
acids, i.e. serine, glycine, alanine and glutamate, increased
significantly in the culture medium. However, their
concentrations varied considerably with algae studied and Fe
addition.