臺灣博碩士論文加值系統

煙麴黴 ( Aspergillus fumigatus Fres. )是普遍存於自然環境中的一種
腐生真菌,會引起人體之過敏及呼吸系統的疾病. 此菌特色在於具有相當
廣的生存溫度範圍, 且為常見的病原菌,在研究中常以其對溫度的感受性,
及二次代謝物的不同而被區分為許多品系. 本研究使用來自世界各菌種中
心對溫度感受性不同之菌株, 及台灣本土所分離出的菌株等共八株,分別
在不同溫度下以 RAPD 分析其遺傳物質的變異及生理狀況表現中,冷及熱
休克蛋白值之差異, 藉以了解在菌類生物中存在的生物多樣性.實驗結果
表示, 利用適當的引子進行 RAPD 的分析, 可以有效且快速地區分種內各
種不同的溫度品系, 且可以將其歸納成群.在冷熱休克蛋白質的研究中,
對高溫抗性高的菌株其熱休克蛋白質在高溫具有較高的表現, 而在一般熱
休克溫度下, 熱休克蛋白質則呈現多樣性. 在冷休克蛋白質方面, 對低溫
抗性高的菌株其表現亦較佳. 若是綜合熱休克蛋白質的圖譜與RAPD 圖譜
一併分析,可以發現兩者的相似性極高. 亦表示熱休克蛋白質的表現與基
因之歧異度具相當程度的關聯. 利用冷休克蛋白質種類, 可以作為區分不
同溫度感受性的品系的方式, 而此種結果也與真菌學上利用營養體生長溫
度之上下限,來區分品系的方式相吻合.

Mitosporic fungi Aspergillus fumigatus Fres. is one of the
wide spreadsaprophytic fungi in our natural natural environment.
It may cause hypersensitive response and disease of respiration
system in human.A. fumigatus is not only a human pathogen but
also an eurytherm. It can be classified into different strains
by the response to tempature or bysecondary metabolism. In this
study, eight different strains with differentresponse to
temperature were collected from several culture centers or
isolated from Taiwan were involed. RAPD analysis, heat-shock and
cold-shockresponses were rested for all strains to find the
difference between strains.The target is to find the differences
in physiology and genetic that show fungal biodiversity.
Using suitable primers, strains can be distinglished different
strains correctly and speedly by RAPD analysis. Expression level
of HSPs at 45Chave variation. Fungal strains adapted in high
temperature( 50 C ) will havestrong HSPs expression. Strains
adapted in lower tempature ( 15 C ) have more CSPs expression.
The comparision of RAPD analysis and HSPs expression resulted
high similarity. On the other hand, expression level of HSPs
hashigh similiatity to genetic biodiversity. Therefore HSPs and
CSPs patternscan also be used to distinguish the strains which
have different thermalsensitivities.