臺灣博碩士論文加值系統

本研究構築兩種果實後熟專一性ACC合成反義基因至pBI121質體中，其一為ACC合成基
因之啟動子與ACC合成反義基因本體間具有180bp反向重複的pBI121-aACC(+)與pBI121-a
ACC(-)；另一則為反向重複序列刪除之pBI121-aACC 1(-)。以香蕉胚性懸浮細胞為受體之
農桿菌(LBA4404)媒介法轉殖，顯示品種、懸浮細胞團大小、農桿菌濃度、共培養光照環
境、懸浮細胞繼代培養日齡、共培養前末次繼代培養之培養基會影響轉殖效率。每克細胞
轉殖率如下：北蕉-pBI121-aACC(-)有22個轉殖細胞系、北蕉-pBI121-aACC(+)有77個轉殖
細胞系、Robusta-pBI121有64個轉殖株系、RobustapBI121有64個轉殖株系、Robusta-pBI
121-aACC(-)有22個轉殖株系。大部分的轉殖株系都具正常的表現型，少部分則出現變異
，從GUS組織染色分析顯示呈鑲嵌的可能性低。從PCR的分析顯示基因已整合入香蕉基因組
中。

Two types of fruit riping specific antisense ACC synthase gene, one with 180b
p invert repeat, pBI121-1ACC(+) and pBI121-aACC(-), and the other one without
the invert repeat, pBI121-aACC 1(-) were constructed. Transformants of banana
were efficiently produced from embryogenic suspension cells cocultivated with
Agrobacterium tumefaciens strain LBA4404 haboring b-glucuronidase, neomycin ph
osphate transferase II and antisense ACC synthase genes. The transformation ef
ficiency was affected by the cultivar of banana, the size of cell cluster, con
centration of Agrobacterium, light condition during cocultivation, subculture
time of suspension cells, and culture medium. The transformation efficiency pe
r gram suspension cells for Pei-Chiao-pBI121-aACC(-) was 22 transgenic cell li
nes, for Pei-Chiao-pBI121-aACC(+) was 77 transgenic cell lines, for Robusta-pB
I121 was 64 transgenic plants, for Robusta-pBI121-aACC(-) was 22 transgenic pl
ants. Most transformants showed normal phenotype and no
chimeric patterns was found. DNA analysis by PCR of the
transformants confirmed the integration of the foreign DNA.