臺灣博碩士論文加值系統

本研究以生物表面活性劑生產菌株 Pseudomonas aeruginosa 4K-1
,為對象 進行最適培養基組成之探討, 主要採用改變單一變數之
方法, 包括碳源、氮源之 種類和濃度、無機鹽類、酵母萃出物濃
度等。結果顯示, 鼠李糖脂之最適生產培 養基組成為: glycerol
5%, NaNO3 0.4%, K2HPO4 0.2%, yeast extract 0.3%, KCl
0.02%, NaCl 0.03%, MgSO4·7H2O 0.02%, MnSO4·H2O 0.001%, ZnSO4
·7H2O 0.00001%, CuSO4 0.00001%。
P. aeruginosa 4K-1 菌株對有機溶劑種類和濃度之耐性研究中發現: 菌
株對 環烷類和異烷類的耐性比同碳數的正烷類要好。隨著正烷類碳
鏈的增長,菌株的耐 性也隨之增加。菌株對長鏈醇類也有不錯的耐
性,而對苯環類化合物的耐性不好, 菌株可以用六個碳以上的正烷
類、八個碳以上的醇類、乙基苯和丙基苯為其碳源 。菌株對有機
溶劑的耐性,除了和菌株本身的構造有密切的關係之外,適當的培養
基組成可改變菌株表面之構造,影響其對溶劑的耐性,也可以增加菌體抵抗
有機溶 劑姿毒害。
脫乳化菌株3-11的培養時間、培養液濃度和溶液酸鹼值與其脫乳化能力有
顯著 之關係。菌株3-11的菌體本身是具有吸附鼠李糖脂的能力, 因
此能夠破壞表面活性 劑所形成的乳化層。加入不同種類和濃度的金
屬鹽溶液會影響菌株3-11脫乳化能 力。
脫乳化劑最適生產培養基組成為: glucose 1.5%, CH3COONH4 0.3%, K2
HPO4 0.2%, yeast extract 0.3%, MgSO4·7H2O 0.02%, CaCL2·2
H2O 0.001%, FeSO4·7H2O 0.001%. 5 升小型醱酵槽之最適
生產條件為: 以2 升的培養液,在培 養溫度30℃,表面通氣,攪拌速
率1vvm的條件下進行生產。

The optimal medium composition for Pseudomonas aeruginosa 4K-1
to produce rhamnolipid were : glycerol 5%,NaNO3 0.4%,
K2HPO4 0.2%, yeast extract 0.3%, KCl 0.02%, NaCl 0.03%,
MgSO4·7H2O 0.02%, MnSO4·H2O 0.001%, ZnSO4·7H2O
0.00001%, CuSO4 0.00001%.
Tolerance level of organic solvents for P. aeruginosa 4K-1 is
much better in cycloalkanes and isoalkanes than in n-
alkanes with the same carbon numbers. Tolerance level in
cell is getting better when n-alkanes with longer
carbon chain. P. aeruginosa 4K-1 have good
tolerance level to long chain alcohols, but not to benzenes .
Cell can assimilate n-alkanes with more than 6
carbons alcohols、with more than 8 carbons、
ethylbenzene and propylbenzene. Tolerance level of
organic solvents depends not olnly on cell structure, but also
depends on medium composition.
Culture times、 de-emulsifier concentration and pH value
influents the de-emulsifying ability of strain
3-11.Strain 3-11 have the ability to adsorb rhamnolipid .
Adding metal salts solution will influent the de-
emulfying ability of strain 3-11.
The optimal medium composition for strain 3-11 to produce
de-emulsifier were :glucose 1.5%, Ch3COONH4 0.3%, K2HPO4 0.2%,
yeast extract 0.3%, MgSO4·7H2O 0.02%, CaCL2·2H2O
0.001%, FeSO4·7H2O 0.001%. The optimal operation
conditions in 5L fermentor were : 2L medium cultures
at 30℃, surface aeration with aeration rate of 1.0
vvm , and agitation at 300 rpm .