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The soybean seed maturation proteins, also known as late embryogenesis abundant (Lea) proteins, are correlated with desiccation tolerance, seed dormancy, abscisic acid (ABA) content, or transition to seedling growth. In this thesis, we characterized soybean seed maturation protein genes in Glycine max and its wild relatives. We also detected the localitation of transcripts of these seven maturation protein genes, via in situ hybridization, in transverse sections of 4 days pod-dried 35 DAF (days after fl Southern analysis, using soybean seed maturation protein cDNA clones as the probes, showed the seven were encoded by single or low copy genes in the genomes from G. max. The data revealed that the insertion or deletion of sequence, and the alteration of restriction sites might be the cause of these polymorphism among the genomes of Glycine. The in situ hybridization, using digoxigenin-labeled antisense transcrips of maturation protein cDNA clones as the probes, showed that the mRNAs of seven maturation protein genes were presented on the different sites in soybean seed tissues. On the transverse sections of 4 days pod-dried 35 DAF seeds, the pGmPM1 mRNA accumulation was detectable in the embryo axis, adaxial epidermis, primary leaves and vascular tissues of cotyledons. The pGmPM2 mRNAs were accumulated in the embryo axis, abaxial epidermis pGmPM4 mRNAs were accumulated in the pith of the embryo axis, primary leaves, metaxylem and phloem of vascular tissues in the cotyledons. The pGmPM5 mRNAs were accumulated both in primary leaves and the parenchyma cells of cotyledons. The pGmPM6 mRNAs were accumulated in all tissues of the seed, except in abaxial epidermis. The pGmPM10 mRNAs were accumulated in the embryo axis, primary leaves, and phloem of vacular tissues in the cotyledons. In addition, the spatial distribution patterns of maturatiin prot
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