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The soybean seed maturation proteins, also known as late
embryogenesis abundant (Lea) proteins, are correlated with
desiccation tolerance, seed dormancy, abscisic acid (ABA)
content, or transition to seedling growth. In this thesis, we
characterized soybean seed maturation protein genes in Glycine
max and its wild relatives. We also detected the localitation of
transcripts of these seven maturation protein genes, via in situ
hybridization, in transverse sections of 4 days pod-dried 35 DAF
(days after fl Southern analysis, using soybean seed
maturation protein cDNA clones as the probes, showed the seven
were encoded by single or low copy genes in the genomes from G.
max. The data revealed that the insertion or deletion of
sequence, and the alteration of restriction sites might be the
cause of these polymorphism among the genomes of Glycine.
The in situ hybridization, using digoxigenin-labeled antisense
transcrips of maturation protein cDNA clones as the probes,
showed that the mRNAs of seven maturation protein genes were
presented on the different sites in soybean seed tissues. On the
transverse sections of 4 days pod-dried 35 DAF seeds, the pGmPM1
mRNA accumulation was detectable in the embryo axis, adaxial
epidermis, primary leaves and vascular tissues of cotyledons.
The pGmPM2 mRNAs were accumulated in the embryo axis, abaxial
epidermis pGmPM4 mRNAs were accumulated in the pith of the
embryo axis, primary leaves, metaxylem and phloem of vascular
tissues in the cotyledons. The pGmPM5 mRNAs were accumulated
both in primary leaves and the parenchyma cells of cotyledons.
The pGmPM6 mRNAs were accumulated in all tissues of the seed,
except in abaxial epidermis. The pGmPM10 mRNAs were accumulated
in the embryo axis, primary leaves, and phloem of vacular
tissues in the cotyledons. In addition, the spatial distribution
patterns of maturatiin prot
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