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研究生:饒芝綾
研究生(外文):Zao, Chih-ling
論文名稱:臺灣地區G2血清型輪狀病毒之基因分析
論文名稱(外文):Genetic Analysis of G2 Rotaviruses in Taiwan
指導教授:李君男李君男引用關係
指導教授(外文):Lee Chun-Nan
學位類別:碩士
校院名稱:國立臺灣大學
系所名稱:醫事技術學系
學門:醫藥衛生學門
學類:醫學技術及檢驗學類
論文種類:學術論文
論文出版年:1997
畢業學年度:85
語文別:中文
論文頁數:61
中文關鍵詞:輪狀病毒
外文關鍵詞:G2血清型rotavirusesG2 serotype
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由臺大醫院1981年至1995年所收集之輪狀病毒檢體分析輪狀病毒在不
同時期流行的血清型分佈情形,發現G2血清型之輪狀病毒在1981年至1987
年都持續有出現,但在這個時期,G2輪狀病毒並非主要盛行的病毒,之後
幾年則未見G2輪狀病毒的檢體出現,直至1992年G2輪狀病毒再度出現,並
在1993年造成大流行。本論文藉由分析病毒株的RNA電泳型,VP7基因之核
酸、胺基酸序列,以及利用RNA雜交試驗以了解G2各病毒株之間的基因變
化與演化關係。 由分析的G2輪狀病毒中,共可定出13種RNA電泳型。
為了解不同電泳型的輪狀病毒間,其基因體之核酸序列是否有所不同,本
研究選擇病毒的中和抗原VP7之基因來做比較分析,從各年代不同電泳型
的檢體中以反轉錄反應及聚合酵素連鎖反應增幅VP7基因,經擇殖並定出
核酸序列,比較病毒株之間的差異,發現核酸序列之相似性達97%以上,
胺基酸序列有差異的位置多落在第1至6的變異區域之內,而1993年流行的
病毒在第96個胺基酸位置的變化將會導致帶電性之改變。經由種系分析,
在本地流行的G2病毒株之間,1992年以後流行的病毒株中,大部份的病毒
和1980年代流行的病毒關係比較遠,另有少數病毒株則和1980年代流行的
病毒較相似。若與參考病毒株比較,在台灣流行的G2病毒株和日本的病毒
株S2比較相似,而和美國、澳洲的病毒株關係較遠。 由RNA-RNA雜交
試驗分析各病毒株間基因的相關性,發現在90年代流行的G2病毒株和80年
代流行的病毒株在第ㄧ、六、七段基因序列的差異較大。將VP1的部份基
因做核酸序列分析,80年代流行的G2病毒株之間VP1基因的核酸序列相似
性為99%,而90年代流行的病毒株之間核酸序列的相似性亦達99%,但80年
代和90年代流行的病毒株間卻只有84%的相似性,顯示這兩個不同時期的
病毒株,在其基因體上已發生相當程度的變異。

Analysis of the serotypes of rotaviruses collected from 1981 to
1995 in National Taiwan University Hospital revealed that G2
rotaviruses were present during the period from 1981 to 1987.
However, G2 rotavirus was not the major prevalent rotavirus
during that period. Later, G2 rotavirus was not detected until
1992, and became the most prevalent rotavirus in 1993. In this
study, genetic and evolutionary relationships among the G2
rotavirus strains were analyzed by electrophoretic profile of
the RNA genome and by comparing the VP7 gene. Furthermore, the
genetic relatedness of the isolated G2 rotaviruses were examined
by RNA hybridization. Thirteen electropherotypes were
identified from the G2 rotavirus specimens. For characterization
of the genetic variation among the G2 strains with different
electropherotypes, the VP7 gene was PCR amplified, cloned, and
sequenced. The homology in the nucleotide sequence among these
rotavirus strains was very high (>97%). Analysis of the deduced
amino acid residues revealed that almost all of the mismatched
amino acid residues were located in the variable regions 1 to 6.
One of the substitutions had a change in charge at amino acid
residue 96. Moreover, the virus prevalent in 1993 had an amino
acid substitution at residue 96 which would have a change in
charge. Phylogenetic analysis had shown that the major G2
rotavirus strains prevalent after 1992 in Taiwan were distantly
related with those prevalent in 1980's. A few strains prevalent
in 1990's were more closely related with the virus strains of
1980's. The major G2 rotaviruses prevalent in Taiwan were more
closed related to Japanese strain, S2, than the other reference
strains, which had nucleotide sequence available, from U.S. and
Australia Genetic analysis by RNA-RNA hybridization had shown
that the first , sixth and seventh RNA segments of the G2
strains isolated in 1990's did not form hybrid bands with those
strains isolated in 1980's. The homology in the nucleotide
sequences of partial VP1 gene was very high (99%) among the G2
strains prevalent in 1980's. Similar result is also shown among
the G2 strains prevalent in 1990's. While there is only about
84% homology between the viruses isolated in 1980's and 1990's.
These data revealed that the virus genome in G2 strains must
have distinct changes between two different periods.

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