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研究生:方家輝
研究生(外文):fang, chia hui
論文名稱:利用酵素反應生產富含n-3及n-6多不飽和脂肪酸的甘油酯
論文名稱(外文):The Production of Acylglycerols Rich in n-3 and n-6 PUFA via Lipase Catalyzed Reactions
指導教授:朱義旭陳特良李文乾陳秀美陳秀美引用關係
指導教授(外文):Chen Teh-LiangLee Wen-ChienChen Kuo-HsiangChung-Hung Lin
學位類別:碩士
校院名稱:國立台灣工業技術學院
系所名稱:化學工程技術研究所
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:1997
畢業學年度:85
語文別:中文
論文頁數:2
中文關鍵詞:琉璃苣油鯡魚油多不飽和脂肪酸酵素亞麻脂酸二十二碳六烯酸
外文關鍵詞:Borage oilmenheden oilPUFAlipaselinolenic aciddocosahexaenoic acid
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本研究於異辛烷中,使琉璃苣油與n-3多不飽和脂肪酸,經IM60(Mucor
miehei脂解酵素固定於陰離子樹脂上)催化進行酯交換反應並嘗試將琉璃
苣油以固定化酵素(Candidarugosa脂解酵素以物理方法,固定於微孔疏水
性聚丙烯擔體上)水解一段時間後,取其甘油酯產物,再加入n-3 PUFA以
IM60催化,進行兩階段的酯交換反應.探討溫度,酵素量,基質濃度及基質重
量比,對酯交換反應的影響.目的在進行油酯的改質,使富含N-6系脂肪酸之
琉璃苣油混入n-3 PUFA,而使甘油酯更具食品醫療的價值.以IM60催化之酯
交換反應,琉璃苣油與n-3 PUFA的基質重量比1:3,基質濃度各為40mg與120
mg,在50C下,反應時間12小時,甘油酯中GLA,EPA及DHA含量可
達20.6%,19.1%,14%.將琉璃苣油以固定化酵素水解4小時,甘油酯中GLA含
量提高,再加入n-3 PUFA,以IM60催化進行兩階段酯交換反應18小時,可得
到甘油酯GLA,EPA及DHA含量為26.5%,19.8%及18.1%.利用固定化酵素水解
鯡魚油,反應時間8小時,可將甘油酯中EPA與DHA含量分別提高至19.4%
與25.6%,取其甘油酯與經由琉璃苣油濃縮後之游離脂肪酸,以IM60催化進
行酯交換反應,在基質重量比1:1時,反應時間12小時,甘油酯中GLA,EPA及
DHA含量可達16.7%,12.8%,19.2%.

In this study,IM60 (Lipase from Mucor miehei immobilized on
anionic resin) was employed to catalyze acidolysis between
borage oil and n-3 polyunsaturated fatty acid (PUFA) obtained
from the saponification of menhaden oil. The acidolysis between
mixtures of acylglycerols obtained from the partial hydrolysis
of borage oil (catalyzed by Candida rugosa lipase immobilized on
microporous polypropylene) and PUFA (derived from menhaden oil)
was also studied. The effects of temperature, enzyme loading,
substrate concentrations, and substrate weight ratio on the
reaction product were investigated. The purpose of this work is
to obtain acylglycerols rich in both n-3 and n-6 fatty acids.For
acidolysis catalyzed by IM60, with concentrations of borage oil
and n-3 PUFA equal to 20mg/ml and 60mg/ml, respectively, and a
reaction temperature of 50℃, the content of GLA, EPA and DHA in
acylglycerols can reach 20.6%, 19.1%, and 14%, respectively.When
borage oil was hydrolyzed for 4h before n-3 PUFA was added to
the acidolysis for another 18h, GLA, EPA and DHA content in
acylglycerols reach 26.5%, 19.8% and 18.1%, respectively.The
hydrolysis of menhaden oil (catalyzed by immobilized Candida
rugosa lipase) yields acylglycerols with EPA and DHA content of
19.4% and 25.6%, respectively, after a reaction time of 8h. The
acidolysis of this acylglycerol and n-3 PUFA derived from
saponification of borage oil (with weight ratio of 1:1) was
catalyzed by IM60 for 12h. GLA, EPA and DHA content in
acylglycerols in the product are 16.7%, 12.8% and 19.2%,
espectively.

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