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研究生:李清祥
研究生(外文):Lee, Chin-Shyang
論文名稱:利用高效能液相層析儀來研究環境中之硫化物對植物Glutathione代謝的影響
論文名稱(外文):To Study the Effects of Sulfate on Glutathione Levels of Water Convolvulus by High Performance Liquid Chromatography
指導教授:陳漢恆陳漢恆引用關係
指導教授(外文):H.H.Chen
學位類別:碩士
校院名稱:文化大學
系所名稱:應用化學系
學門:自然科學學門
學類:化學學類
論文種類:學術論文
論文出版年:1997
畢業學年度:85
語文別:中文
論文頁數:68
中文關鍵詞:硫化物植物代謝
外文關鍵詞:GlutathioneSulfate
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本研究以生長期30天的空心菜在純水及0.05M的硫酸鈉溶液,不同時
間(0,24,48,72,96小時)的培養,測空心菜Glutathione含量,用來探討環境
中硫化物對植物組織硫代謝的影響, 本實驗結果指出,當空心菜在濃
度0.05M之硫酸鈉,其Glutathione含量隨著時間之增加而增加的趨勢,在培
養72小時,空心菜Glutathione 含量達最高(增加152%),隨後96小時,空心
菜生長情形逐漸受損,Glutathione增加百分比逐漸下降(123%).而在純水
中培養之空心菜,Glutathione的含量會隨著培養時間的增加而下降,於培
養24,48,72,96小時後,Glutathione的含量分別為88%,80%,73%,69%.若
以0.02M硫酸鹽溶液,經過24,48,72,96小時後,空心菜所得之Glutathione
含量分別定為100%,受0.1M培養所得之Glutathione含量,隨培養時間的增
加(24~72 hours),Glutathione含量為0.02M的1.5倍,但96小時之後,下降
為1.2倍.增加百分比由初期的迅速增加而後百分比增加率會逐漸減緩.(參
考吳幸玲,1997) 空心菜和菠菜,經由純水和0.1M硫酸鹽溶液培養,葉部組
織Glutathione含量之比較(參考黃北緯,1996).以30天空心菜之
glutathione含量為100%(151.8nmole/FGW),與20天菠菜Glutathione含量
相近(176.1nmole/FGW),45天菠菜Glutathione含量約為空心菜之2倍.但在
純水培養至96小時後,20天的菠菜的Glutathione含量百分比,出現+-20%的
變化,45天菠菜則呈現+-10%的變化,顯示菠菜因應培養環境之改變,會有較
大的Glutathione生合成及包函的適應能力.另外,在0.1M硫酸鹽溶液培
養24~96小時後,30天空心菜與20天菠菜之Glutathione變化值相近;45天的
菠菜,由於菠菜其根部對硫酸鹽吸收能力較空心菜強,轉化為Glutathione
亦相對來的多,45天的菠菜,為30天空心菜Glutathione含量增加約2倍.本
研究是利用高效能液相層析儀(HPLC)來測定植物葉中Glutathione的含量.

The objective of this thesis is to study the effects of
pure water and 0.05M sodium sulfate on the glutathione levels of
water convolvulus. The water convolvulus of 30 days ages was
cultivated in pure water and 0.05M sodium sulfate solution at
different times (0,24,48,72,and 96 hours)and determined the
levels of glutathione in leaves, respectively. The results found
that there is a correlation between the levels of glutathione in
water convolvulus and the cultivated sodium sulfate
concentration up to72 hours . The levels of glutathione in the
leaves of 0.05M sodium sulfate treated water convolvulus at 72
hours and are approximately 1.5 times higher than the
corresponding control value. The increases of glutathione
dropped gradually thereafter,due to the effect of sodium sulfate
on the growth deterioration of water convolvulus. The levels of
glutathione of water convolvulus leaves which cultivated in pure
water decrease as the times increase.The Glutathionelevels of
water convolvulus in 0.02M sulfate solutions at different time
intervals treated as 100%. At 0.1M sulfate solutions, the
Glutathione levels from 24 to 72 hours increased 1.5 times; then
drop to 1.2 times at 96 hours. Those results show that the rate
of biosynthesis of Glutathione ishigher up to 72 hours if
cultivated at 0.1M sulfate solution. Both species cultivated in
pure water and the Glutathione levels of water convolvulus for
different time intervals treated as 100%. The growing trends of
corresponding Glutathione levels of 20 and 45 days old spinach
drop gradually from 116% to 75.9% and from 230.2% to
198.6%,respectively as times increase. These results show that
Glutathione levels of 20 and 45 days old spinach has
approximately +-20% and +-10% wider ranges of depletion,
respectively as compared with water convolvlus. Both species
cultivated in 0.1M sulfate solution and the Glutathione levels
of water convolvulus at different time intervals treated as
100%. The growing trend of corresponding glutathione of 20 days
old spinach shows similarly as water convolvulus. The growing
trend of Glutathione of 45 days old spinach decline gradually as
times increasa. These results show that nature spinach may not
need to increase the biosynthesis of Glutathione similarly as
water convolvulus even cultivated at 0.1M sulfate solution. The
leaf Glutathione levels were determined by high performance
liquid chromatograpry.

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