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研究生:吳雪華
研究生(外文):Wu, Hsueh-Hua
論文名稱:核酸混合物對小白鼠記憶能力及腦神經傳導物質之影響
論文名稱(外文):Effects of nucleoside-nucleotide mixture on memory and brain neurotransmitter in mice
指導教授:王銘富王銘富引用關係
指導教授(外文):Wang Ming-Fu
學位類別:碩士
校院名稱:靜宜大學
系所名稱:食品營養學系
學門:醫藥衛生學門
學類:營養學類
論文種類:學術論文
論文出版年:1997
畢業學年度:85
語文別:中文
論文頁數:2
中文關鍵詞:核酸混合物老化促進小白鼠單次被動迴避試驗學習與記憶單胺
外文關鍵詞:nucleoside-nucleotide mixturesenescence accelerated mousesingle-trial passive avoidance testlearning and memorymonoamine
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阿滋海默氏症是一種持續且無法回復的腦部退化性疾病,主要特徵
為記憶力逐漸減退。 細胞外之核酸混合物會刺激星狀細胞增殖,並可
合成神經生長因子,而增加齧齒動物腦中神 經生長因子之濃度,可改
善因年老所導致之記憶缺損。本研究之目的在探討飼料中添加核酸 混
合物對記憶缺損小白鼠之記憶能力改善,並分析腦中神經傳導物質之變化
情形。實驗利用 1及7月齡雄性老化促進小白鼠、1月齡雄性ddY系及低
學習性小白鼠。各分成兩組,分別餵飼 20﹪酪蛋白(對照組)及20﹪
酪蛋白中添加0.5﹪核酸混合物。餵飼實驗飼料3週後,測各組 小白鼠
平面步移之行為活動情形,且於攝食實驗飼料4、8與12週後,分別以單次
被動迴避試 驗測試小白鼠之學習與記憶能力。行為測試結束後,將小
白鼠犧牲取腦之皮質部、海馬迴、 紋狀體與黑質四部份腦區,將其研
磨、均質、離心及過濾後,利用微分離管高效液相層析儀 -電化學偵
測器進行腦部單胺類神經傳導物質及其代謝產物之分析。
結果顯示各品系之兩組小白鼠在實驗期間之體重、攝食量與活動量皆無顯
著性之差異。 在學習記憶能力方面,SAM-P/8系1月齡小白鼠餵飼核酸
混合物,其影響作用並不顯著;而 SAM-P/8系7月齡小白鼠在餵飼實
驗飼料第12週,測其24小時記憶能力平均值比對照組有顯著 性提高。
ddY系小白鼠於早期腦成長快速發育時,添加0.5﹪核酸混合物有增強記憶
之作用; 而添加0.5﹪核酸混合物對PLM系小白鼠在第8及12週其學習
記憶能力均比對照組有較好的傾 向。另外,在腦部神經傳導物質方
面,實驗組比對照組小白鼠腦中單胺及其代謝產物之含量 在大腦皮質
、海馬迴、紋狀體與黑質四部份腦區,大都呈現增加之傾向。
由本實驗結果得知,在飼料中添加0.5﹪核酸混合物有某種程度上改善記
憶缺損小白鼠之 學習記憶能力,而在腦部單胺及其代謝物質含量方面
,亦有減少腦神經傳導物質降低之作用 ,但是其間之關連性還需再進
一步之探討。
Alzheimer''s disease is a continued and incapable of
restoring brain neurodegenerative disease, which causes memory
impairment. Extracellular nucleosidesand nucleotides can
stimulate proliferation of astrocytes, nerve growth factor (NGF)
synthesis, and increase the concentration of NGF in brain, which
ameliorate age-related memory deficits in rodents. We studied
the effect of dietary nucleosidesand nucleotides mixture (NNM)
on memory deficient mice, and analyzed the brain
neurotransmitter in mice. The experiment used 1 and 7 month old
male senescenceaccelerated mice (SAM), 1 month old male ddY and
poor learning mice (PLM). Theywere separated to two groups, one
group was fed with 20% casein diet (control),the other fed with
20% casein diet supplemented with 0.5% NNM for 12 weeks.
Theambulatory activity of the mice was measured after 3 weeks,
and memory test wasstudied by a single-trial passive avoidance
test after 4,8, and 12 weeks. After finishing behavioral test,
mice were killed, each brain was dissected into the four brain
regions (cortex, hippocampus, striatum and substantia nigra),
tissues were homogenized, after centrifuged and filtrate was
directly injected into the microbore column high performance
liquid chromatography-electrochemical detection(microbore HPLC-
ECD) system and the metabolites of monoamine
neurotransmitterwere be assayed. The results show that body
weight, food intake and locomotion of the mice areabout the same
between the two groups. In those memory test, there are not
significanted effect on 1 month old SAM-P/8 strain mice fed with
supplemented 0.5% NNM. For the 7 month old SAM-P/8 strain mice
fed with supplemented 0.5% NNMafter 12 weeks, we measured the
average time of successful memory 24 hours after the punishment
and we found it significantly higher than the control group
(p<0.05).In the ddY strain mice during the development of early
brain period, the diet withsupplemented 0.5% NNM that can
enhance memory ability. Whereas the diet supplementedwith 0.5%
NNM for 1 month old PLM mice after 8 and 12 weeks was better
than thecontrol group. Moreover, the level of the monoamine and
their metabolites increasein cortex, hippocampus, striatum and
substantia nigra of the experimental mice,compared with the
control group mice. To sum up, the present finding is
supported by the dietary administration of 0.5% NNM, that
improves in the ability of memory deficient mice, and decreases
the decay of brain monoamine neurotransmitter and their
metabolites. Furthermore,the relationship between memory
improvement and the brain monoamine neurotransmitterwould need
explored in the future.
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