(18.206.177.17) 您好!臺灣時間:2021/04/23 05:44
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:王昭凱
研究生(外文):Wang, Chao-Kai
論文名稱:以植入S.D.rat*s大腿肌肉模式評估豬皮膠原蛋白膜誘發之組織病理變化與免疫反應
論文名稱(外文):Assays of Histopathologic Change and Immune Response Induced by Porcine Dermal Collagen Membrane, Evaluated by S.D. Rats Animal Model
指導教授:呂炫Kun
指導教授(外文):Lu Hsein-Kun
學位類別:碩士
校院名稱:台北醫學院
系所名稱:口腔復健醫學研究所
學門:醫藥衛生學門
學類:牙醫學類
論文種類:學術論文
論文出版年:1997
畢業學年度:85
語文別:中文
論文頁數:67
中文關鍵詞:豬皮膠原蛋白膜戊二醛免疫生成性
外文關鍵詞:porcine dermal collagen membranegluraraldehydeimmunogenicity
相關次數:
  • 被引用被引用:0
  • 點閱點閱:108
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
本研究之目的為針對已改良之豬皮膠原蛋白膜(porcine dermal
collagen membrane--PDCM),經四種不同濃度(0%,0.01%, 0.05%, 3%)之
戊二醛(GA)交鏈一小時後之成品,以Sprague-Dawley rats(S.D. rats)
實驗動物模式植入其肌肉層內,經 1,3,5,7,10,14,21,28,42日,共九個
階段後,利用傳統 Hematoxylin & Eosin stain(H-E stain), Alcian
blue-PAS(AB-PAS) stain,及免疫染色等技術,評估豬皮膠原蛋白膜之
活體吸收速率及所引發組織病理變化與免疫生成性(immunogenecity)。結
果發現:PDCM之活體吸收速率:0% GA-PDCM<三週內被吸收0.01% GA-
PDCM<六週內被吸收0.05%及3% GA-PDCM>六週內仍未被完全吸收。
而PDCM維持細胞隔離效果之時間則為: 3% GA-PDCM可維持21日細胞隔離
;而0. 05%、0.01%、0% GA-PDCM 僅可維持七∼十日 就被細胞及新生微
血管增生、穿透。 在組織病理變化方面:豬皮膠原蛋白膜會誘發S.D.
rats出 現慢性異物反應,並逐漸發展為DTH反應;其激烈程度隨 著膜片
存在時間延長而加劇,亦隨著膜片被吸收而減緩。 亦即為3%GA-PDCM之
異物反應最持久且能發展較為較 成熟的DTH反應。 而經由免疫染色證實
:豬皮膠原蛋白膜確實會誘發以 macrophage 主導之DTH反應。且此反應
與CD4 T lymphocytes 相關,而與CD 8 T lymphocytes 無關;且植 入
區域內之肌肉及修復膜片之結締組織並未出現抗體 (IgG,IgM)大量沉積於
此處,表示PDCM 誘發type II hypersensitivity 之可能性極低。 因
此推測PDCM所引發之免疫反應屬於Thl cell主導之反應。若應用PDCM於牙
周治療,則除了可能具有引導組織再生之效果外;且可能以Th1 cell 分
泌之IL-2、IFN-r等cytokines,使發炎牙周組織朝向Thl主導之組織修復
的方向進行?
The histopathologic change and immune response, induced by
porcine dermal collagen membrane(PDCM), was evaluated by
Sprague-Dawley rats animal model. The PDCM, which was cross-
linked by 3%, 0.05%, 0.01%, or 0% glutaraldehyde (GA) for 1
hour, was implanted into muscle tissue of S.D. rats. Following
1, 3, 5, 7, 10, 14, 21, 28, 42 days, the rats were sacrificed;
PDCM with the surroundingmuscle tissue in implanted sites were
prepared for (1) Hematoxylin & Eosin stain, (2) Alcian blue-PAS
stain, (3) immunohistochemistry stain(avidin-biotin complex
method). According to microscropic observation, the resorption
rates of PDCM in vivo were: 0% GA-PDCM less than 3 weeks,
0.01% GA-PDCM less than 6 weeks, 0.05% and 3% GA-PDCM
longer than 6 weeks.However, the sustenance to maintain cellular
seperating effect was: 3% GA-PDCM maintaining cellular
seperating effect for 21 day, and 0.05%、0.01%、0% GA-
PDCM maintaining for 7 to 10 days. After this period,
PDCM were invaded by fibroblasts and capillaries. PDCM could
induced foreign body reaction in implanted muscle tissue, which
would progressed to delayed type hypersensitivity (DTH). The
intensity of induced DTH was corelated to the persistence of
PDCM; the longer PDCM persised , the more intensively the DTH
would be. The DTH induced by 3% GA-PDCM was most intensively and
progressed to most mature reaction, but those of 0%GA-PDCM was
most mild. According to the result of immunohistochemistric
stain, the activated macrophages were the primary effector cells
of DTH. This reaction was corelated to CD4 T lymphocytes, but
not to CD8 T lymphocytes. In implanted sites, there was no
significant amount of IgG、IgM antibody deposition. In other
words, the possibility of PDCM inducing type II hypersensitivity
was very low. According to above finding, we suggest that the
immune response induced by PDCM belongesto reaction managed by T
helper 1(Th 1) cells. In the furture, if PDCM is utilized in
periodontal therapy, there would be not only guded tissue
regenerative effect, but also changing immune response from
destructive pathway to repair one,which was managed by Th 1
cells.

QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
系統版面圖檔 系統版面圖檔