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研究生:陳繼勝
研究生(外文):Chen, Chi-Sheng
論文名稱:大白鼠大腦體感覺皮質桶狀排與桶狀弧間之功能重組:碳十四去氧葡萄糖之研究
論文名稱(外文):Functional reorganization between barrel row and barrel arc in rat first somatosensory cortex: A 14C-2-deoxyglucose study
指導教授:許世昌許世昌引用關係
指導教授(外文):Sheu, Shyh-Chang
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:解剖學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:1997
畢業學年度:85
語文別:中文
論文頁數:69
中文關鍵詞:解剖解剖科學皮質桶狀排碳十四葡萄糖
外文關鍵詞:ANATOMYANATOMICAL-SCIENCE
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在囓齒類動物的顎部有五排顯而易見的觸鬚。對這類動物而言,它們是相當重要的感覺器官,與觸覺的分辨、方向感的辨認及協助行動與平衡方面有著莫大的關係。根據過去解剖學、電生理學及放射性碳十四去氧葡萄糖(l4C-2-deoxyglucose;2DG)等方法的研究結果顯示,每一根觸鬚會以一對一的體區位排列(somatotopic arrangement)方式投射到對側大腦初級體感覺皮質桶狀皮質區(barrel cortex)第四層中的桶狀結構(barrel)。
由過去的研究發現,若將C3(C排第3根)觸鬚除外的所有觸鬚毛囊全部去除,則C3觸鬚除了投射到正常所應投射的大腦皮質C3桶狀結構外,尚會擴大投射到四周被去除觸鬚所相對應的桶狀結構上,顯示有發生同排間及跨排間的功能重組。此外,僅去除除了C3觸鬚外的C排觸鬚,則有發生同排間的功能重組;但若僅去除除了C3觸鬚外的第3個觸鬚弧,卻沒有出現跨排間的功能重組。因此,何種型式的局部觸鬚毛囊去除才會出現跨排間的功能重組便是本實驗所欲探討的問題。
本實驗將大白鼠(n=30)於出生後第六天(postnatal day6;PND6),去除其左顎部特定的觸鬚毛囊,右側則作為對照。依據左顎部觸鬚毛囊去除的形式,將實驗動物分為以下五組:(1)去除所有的觸鬚毛囊;(2)去除B、D兩排的第1、2及3根觸鬚毛囊;(3)去除B、D兩排的第3、4及5根觸鬚毛囊;(4)去除B、D兩排的第2、3及4根觸鬚毛囊;(5)去除B、D排的第1、2、3、4及5根觸鬚毛囊。
經3個月存活期後,便進行2DG實驗。首先同時刺激兩側的C3觸鬚(第一組不進行刺激),並經股靜脈給予2DG,此後的45分鐘過程中進行採血,之後經心臟灌流固定後,取出大腦做冷凍切月,利用腦組織切片製成自動放射顯影X光片,而腦組織切月則進行細胞色素氧化酵素(cytochromeoxidase;CO)染色,以定位出桶狀結構,再運用MCID影像分析系統對X光片加以分析,以探討大白鼠大腦桶狀皮質中桶狀排與桶狀弧間功能重組的情形。
從組織染色片中觀察得知,所有動物實驗側的桶狀結構與對照側相類似,並不因觸鬚毛囊被去除而有所改變。而由X光片的分析可以發現,第一組中,實驗側與對照側的葡萄糖基礎代謝率相近。而在第二、三組中,實驗側與對照側除了C3桶狀結構的葡萄糖代謝率升高外,其餘的桶狀結構則僅維持著基礎代謝率,並沒有引起跨排的桶狀結構增加對葡萄糖的代謝率。而在第四組中,可發現到實驗側葡萄糖代謝率增加的區域除了正常投射的C3桶狀結構外,尚擴大至部份的B3及D3桶狀結構,顯示有跨排間的功能重組現象。在第五組中,實驗側葡萄糖代謝率增加的範圍除了正常投射的C3桶狀結構外,尚擴大至大部份的B3及D3桶狀結構及部份的B2、B4、D2及D4桶狀結構,顯示跨排間功能重組的情形比第四組者更為明顯。 綜合以上結果顯示:(1)大白鼠於出生後第六天所進行的觸鬚毛囊去除手術,並不會使其大腦皮質桶狀結構之形態產生明顯的變化:(2)去除觸鬚毛囊並不會改變與其對應之桶狀結構的葡萄糖基礎代謝率;(3)在特定觸鬚毛囊被去除的同時,若其同排且相鄰的觸鬚毛囊亦被去除,則其鄰排且相鄰的觸鬚-皮質桶狀結構系統會出現跨排的功能重組。
The mystacial whiskers of rodents are organized in five distinct rows. The sensory function of the whisker may be related to tactile discrimination, orientation, locomotion and balance. A one-to-one relation between a mystacial whisker and its corresponding cortical barrel was suggested by anatomical, electrophysiological and 14C-2DG findings.
Stimulation of the spared C3 whisker produced in an enlarged pattern of 2DG labeling in first somatosensory (SI) cortex. This result revealed that the functional reorganization may occurred across the barrel row after spared C3 preparation. On the other hand, there was no functional reorganization occurred across the barrel row after 3rd arc of whisker follicles ablation. Thus, the purpose of the present study was to examine the functional reorganization between the barrel row and barrel arc following specific pattern of whisker follicles ablation.
Thirty Sprague-Dawley rats were used in this study. Unilateral whisker follicles ablation of all whiskers (group1); B1-B3 and D1-D3 (group2); B3-B5 and D3-D5(group3); B2-B4 and D2-D4(group4); and B1-B5 and D1-D5 (group5) were performed on postnatal day 6. After three months of survival, 2DG was injected intravenously and the C3 whisker brush stroked bilaterally (no stimulation in group1 rats) for 45 minutes and blood was sampled during this period. Animals were perfused through heart and brain removed for later cryostat sectioning. Autoradiograms and cytochrome oxidase (CO) stained brain sections of the SI cortex were analyzed using a MCID (microcomputer imaging device) system.
From the observations of the CO stained brain sections reveal that the morphology of the barrel in experimental side was similar to that of the control side, and not affected by the follicles ablation. The findings from the analysis of the autoradiograms were as follows. In group1, the basic metabolic rates of glucose in both sides of the barrel cortex were similar. In other groups, stimulation of control C3 whisker only increases the 2DG labeling on contralateral C3 barrel. In group 2 and 3, the increased 2DG labeling on both sides were similar. In group 4, the increased 2DG labeling on experimental side was expended to the B3 and D3 barrels. In groups, the increased 2DG labeling on experimental side was more obvious than group 4, it expended to the B3 and D3 barrels or even to the B2, B4, D2 and D4 barrels.
In conclusion, (1) removal of whiskers on postnatal day 6 do not cause any apparent cytoarchitectural changes in the barrel field; (2) the basic metabolic rates of glucose in barrel cortex do not affected by the follicles ablation; (3) the functional reorganization may occurred across the barrel row after specific pattern of whisker follicles ablation.



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