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研究生:李元民
研究生(外文):Lee, Yuan-Min
論文名稱:馬偕醫院門診孕婦之弓蟲抗體、抗原篩檢
論文名稱(外文):Screening on antigens and antibodies of toxoplasma gondii among pregnant women from outpatient department of Mackay Memorial Hospital
指導教授:蔡洪又欽
指導教授(外文):John TsaiHong
學位類別:碩士
校院名稱:國立陽明大學
系所名稱:寄生蟲學研究所
學門:醫藥衛生學門
學類:醫學學類
論文種類:學術論文
論文出版年:1997
畢業學年度:85
語文別:中文
中文關鍵詞:寄生蟲學弓蟲感染
外文關鍵詞:PARASITOLOGY
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弓蟲是一種寄生性原蟲,它可感染懷孕婦女,再經由胎盤感染胎兒,會導致嬰兒中樞神經系統之異常,稱之為先天性弓蟲症。本實驗以馬偕醫院產科門診之孕婦為研究對象,探討弓蟲特異性抗體在此一族群之陽性率,並評估以循環性抗原來偵測弓蟲症之可行性。弓蟲特異性抗體之篩檢以酵素結合免疫吸附法測試,並利用間接螢光抗體測試確認,受檢2,100名孕婦中307名(14.62%)被檢出有弓蟲特異性抗體lgG,其螢光抗體之陽性力價分別為1:32者佔207名(9.86%);1:64者佔42名(2.0%),1:128者佔17名(0.81%);1:256者佔15名(0.71%);1:512者佔25名(1.19%);1:1024者佔1名(0.05%)。弓蟲循環性抗原之檢測,以免疫墨點法進行初步篩檢,並以酵素結合免疫吸附法進行確認,從2,100件孕婦血清中有3件被檢出帶有弓蟲抗原。進一步以弓蟲42KD或130KD蛋自之免疫血清進行測試時,相同之3名受試者血清亦呈現陽性反應。42KD或130KD蛋自以螢光抗體法發現其主要分佈於弓蟲體表之前端及末端,而42KD在弓蟲體表中心也有分佈;兩者均為含醣類不含脂質之蛋白。以衍生的特異性抗體與其他不同種之血液內寄生蟲(日本血吸蟲、曼氏血吸蟲、犬焦蟲)產生之循環性抗原反應,並無交叉反應。綜合以上結果,瞭解循環性抗原產生的機轉及抗原特性,對未來弓蟲的偵測及疫苗發展與防治必能有所幫助。
Toxoplasma gondii is a parasitic protozoan. After acquiring the infection during pregnancy, this parasite may be transmitted to the fetus through the placenta causing congenital toxoplasmosis. The infected fetus may have abnormalities in the central nervous system after birth. This study was conducted to determine the sero- prevalence of toxoplasmosis among the pregnant woman participating the prenatal examination in the Department of Obstetrics of Mackay Memorial Hospital. Moreover, it was also designed to assess whether it is possible to diagnose this infection by detecting the circulating antigens of this parasite. By using the enzyme -linked immunosorbent analysis (ELISA) and confirmed by the indirect fluorescent antibody test (IFA), specific lgG against T. gondii were detected in 307 of 2,100 samples collected, the overall sero-prevalence was 14.62%. Among them, 207 (9.86%)with titer at 1:32, 42(2.0%) with titer at 1:64, 17(0.81%) with titer at 1:128, 15(0.71%) with titer at 1:256, 25(1.19%) with titer at 1:512, and 1(0.05%) with titer at 1:1024. In the primary screening, circulating antigens ofT. gondii were detected in 3 samples (0.14%,3/2,100) using the immuno-dotting technique and confirmed by ELISA. These positive samples also yielded positive results in the IFA tests using 42 KD or 130 KD anti-T. gondii antibodies. The 42 KD and 130 KD antigens mainly distributed at the apical complex and on the posterior surface of the parasite. In addition, the 42 KD antigen coiiJd also be detected in the interior of the protozoa. Biochemically, these two antigens were proteins containing carbohydrates but without lipid components. The antibodies against these antigens did not cross-reacted with the circulating antigen from some blood parasites (Schistosoma japonicum, S. mansoni, and Babesia gibsoni). However, understanding the mechanisms in the production of the circulating antigens and their antigenic properties is helpful to the diagnosis of toxoplasmosis and the development of vaccine against this parasite.



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