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研究生:謝鳳龍
研究生(外文):Hsieh- Feng-Long
論文名稱:利用紅麴菌以固-液態培養方式產生膽固醇合成抑制劑-
論文名稱(外文):Production of Hypocholesterolemic Agent by Monascus pilosus in Solid-Liquid Culture
指導教授:張耀南張耀南引用關係
指導教授(外文):Chang Yan-Naw
學位類別:碩士
校院名稱:大葉大學
系所名稱:食品工程研究所
學門:農業科學學門
學類:食品科學類
論文種類:學術論文
論文出版年:1998
畢業學年度:86
語文別:中文
中文關鍵詞:紅麴菌回應曲面法固液態培養膽固醇合成抑制劑
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本研究以 Monascus pilosus CCRC 31527 為主要紅麴生產菌株, 進行最適搖瓶培養條件及培養基碳、氮源等因素之探討。 結果顯示最適培養溫度介於 25-30 ℃之間,而最佳培養基起始酸鹼值及其體積含量亦隨溫度而有所改變。在 25 ℃培養下,培養基的最佳起始酸鹼值為 pH 8,最適體積含量為 25 ml,可達產量 1.54 × 10-3 mg/ml;而在 30 ℃培養時,培養基最佳起始酸鹼值為 pH 5,體積含量為 125 ml 最適合。 另外,最佳澱粉類或醣類碳源為白米澱粉,有機氮源會隨培養溫度而有所不同,在 25 ℃培養下,酵母萃取物為最佳者;而 30 ℃下,則以 peptone 為佳。 在 25 ℃溫度下培養 ,以白米為澱粉類碳源時,在 25 ml 的培養基體積中, 使其起始酸鹼值為 pH 8.0的固 - 液態培養條件下, 可使本研究 monacolin K 產量達到最高, 約為 7.178 ×10-3 mg/ml。 可知,以固 - 液態培養培養方式提高膽固醇合成抑制劑產量是值得探討的。 另外,利用回應曲面法尋求紅麴菌生產膽固醇合成抑制劑之最適化培養條件時,發現酵母萃取物的濃度設計,應偏向於愈低濃度的設計,中心點設為 0.5 g/L;甘油則要偏向高濃度設計, 中心點設為 120 ml/L,其他如白米澱粉、葡萄糖等的濃度則不作改變,如此才可能獲得較好之結果。
In this study, monacolin K was produced by Monascus pilosus CCRC 31527in solid-liquid culture. Several different strategies of manipulatingvariables, such as culture temperature, initial pH and volume of medium,carbon and nitrogen source, were investigated. It was found that the optimumculture temperature was between 25 oC and 30 oC. The optimum media in theculture of 25 oC were found to be pH 8.0 and 25 ml; while those in theculture of 30 oC were to be pH 5.0 and 125 ml. Furthermore, rice powder wa sconsidered to be the best carbon source in giving the maximum yield ofmonacolin K. The maximum yield was 7.178(10-3 mg/ml in the culture of 25 oC.The appropriate organic nitrogen source was changed with the culturetemperature. Among the nitrogen sources tested, yeast extract and peptonewere found to be suitable for monacolin K production in the culture of 25 oCand 30 oC, respectively. This research demonstrated that the solid-liquidculture was worth improving the yield of monacolin K. Response surface methodology was used to optimite monacolin K productionby M. pilosus CCRC 31527 in flask culture. Analysis of variance indicatedthat the interaction between operation variables (yeast extract andglycerin) in the quadratic model was only significant. The concentration ofyeast extract at the design center point was recommended to be at 0.5 g/L,while the concentration of glycerin was at 120 ml/L and the concentrationsof both rice starch and glucose were 30 g/L. For this kind of complexmedium, it would be good for monacolin K production by M. pilosus CCRC31527.
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