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When analyzing the regression model with nested-error structure, if the correlations between errors are ignored, and conducting the model adequacy test by the standard F statistic (Fs) led from the ordinary leastsquares estimator (OLSE), then the type I error rate will be inflated. However, if the correlated structure is considered and the model is tested by FGLS led from the general least-squares estimator (GLSE), the calculation will be more complicate. The model can be transformed to a new model with independent rando errors and then, tested by Fs. The result is the same as the one by FGLS, also it is more convenient for calculation. Since the transformation matrix is a function of variance components, we estimate variance components by Henderson's fitting-of-constants when they are unknown. Through simulation, it is concluded that if the observations in each stage of nested-error structure are the same, the GLSE is more stable than the OLSE in both two-stage and three-stage structures. Also, the power and the sizes of FGLS will perform better than those of Fs.
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