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研究生:李平南
研究生(外文):Lee, Pin-Nan
論文名稱:短期維生素E注射對熱季荷蘭牛抗氧化活力及泌乳功能之影響
論文名稱(外文):Effects of short term vitamin E injection on antioxidative capacity and lactation performance of Holstein cow in hot season
指導教授:張釵如
指導教授(外文):Chai-Ju Chang
學位類別:碩士
校院名稱:國立中興大學
系所名稱:畜牧學系
學門:農業科學學門
學類:畜牧學類
論文種類:學術論文
論文出版年:1998
畢業學年度:86
語文別:中文
論文頁數:101
中文關鍵詞:維生素E乳牛抗氧化泌乳
外文關鍵詞:Vit ECowAntioxidationLactation
相關次數:
  • 被引用被引用:6
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短期維生素E注射對熱季荷蘭牛抗氧化能力及泌乳功能之影響李 平 南摘
要維生素E(vitamin E, vit E)為動物體中最重要的抗氧化微量養份之一
,本實驗在短期內(5天)額外注射(i.m.)乳牛以高單位之vit E (3000IU/
d),並且分析注射前(pre-)、後(post-)、及注射後一週(7d post)乳牛血
漿、紅血球及乳汁中維生素E含量及抗氧化酵素活性之變化情形,以評估
vit E對於乳牛體內抗氧化因子及乳腺生理之影響,試驗vit E注射是否可
以改善乳牛體內之氧化-抗氧化平衡。主要試驗結果:在三個採樣階段,乳
牛血漿中、紅血球中及乳汁中之vit E濃度在post-皆有明顯之升高(
p<0.05),提高之倍數約為對照組之3.3、1.2及2.3倍;在7d post時,vit
E濃度又下降,與對照組無顯著差異。在血漿一般成份,LDH(lactate
dehydrogenase)及CK(creatine kinase)在post-明顯升高,其原因可能係
為注射之肌肉傷害所導致,而乳汁之一般成份則無顯著差異。血漿中之
SOD(superoxide dismutase)、CAT(catalase)及GSH-Px(glutathione
peroxidase)活性在vit E注射前後皆無明顯變化,但在紅血球中,GSH-Px
於post-時活性下降(p<0.05),SOD及GSH-Px則無明顯變化;乳清中之抗氧
化酵素活性亦沒有明顯變化。Vit E處理組之血漿及乳汁內之抗氧化能力
在post-皆較pre-時為高,而在7d post又下降,但仍較pre時為高。在實
驗數據之相關性:乳牛血漿中vit E濃度對血漿中CREA(reatinine)、TG(
triglyceride)及CK(creatine kinase)無顯著相關,但與CHOL(
cholesterol)及LDH有極顯著正相關(0.49及0.52,p<0.01);紅血球中之
vit E與紅血球中GSH-Px之相關性為-0.48(p<0.05)。乳汁中vit E濃度與
乳清中SOD、GSH-Px、及CAT無顯著相關,但SCC(somatic cell count)與
三者皆有極顯著正相關(0.76、0.78及0.69,p<0.01);乳中SCC與乳中TP(
total protein)呈正相關(0.57,p<0.01),但與lactose成顯著負相
關(-0.61,p<0.01)。根據以上之結論:肌肉注射vit E(3000 IU/d,連續5
天)對乳牛血漿、紅血球及乳汁中之vit E有明顯提高之作用,並且提高血
中及乳中之抗氧化能力。試驗處理對肌肉傷害及緊迫指標(如CREA、LDH、
CK)有升高之現象,對其他乳牛之血漿及乳汁中一般成份無顯著性之影響
。Vit E對於乳牛各組織中抗氧化酵素活性並未顯出很大之影響。另外,
乳中抗氧化酵素活性之存在,推測來自於血液或乳汁之體細胞。
Effects of short term vitamin E injection on antioxidative
capacity and lactation performance of Holstein cow in hot
seasonPin-Nan LeeSummary Vitamin E(vit E) is one of the most
important antioxidative micronutrients in animals. The purpose
of this study was to investigate the effects of short term vit E
injection(3000 IU/d, for 5 day)(i.m.) on the major milk
components, plasma metabolic contents, and milk, RBC and plasma
antioxidative enzyme activities and their vit E contents of
lactating Holstein cows. Twelve lactating cows were randomly
allocated into two groups: placebo injection as control and vit
E injection. Blood and milk samples were collected at day 0
(before vit E and placebo injection, pre-), day 5 (after
injection, post) and day 12 (one week after injection, 7d post).
The results suggested that the post vit E concentrations of
plasma, RBC and milk serum were significantly elevated by 3.3,
1.2 and 2.3 folds in the vit E treated cows as compared to those
of placebo-treated cows (p<0.05), levels then declined at 7d
post. Plasma LDH (lactate dehydrogenase) and CK (creatine
kinase) activities were significantly elevated after placebo and
vit E injection. No significant differences in plasma SOD
(superoxide dismutase), CAT (catalase) and GSH-Px activities
were found at all pre-, post- and 7d post stages between placebo
and vit E-treated cows, and neither did SOD and CAT activities
in RBC. RBC GSH-Px activity was decreasd significantly (p<0.05)
in vit E injection cows in comparison with placebo-treated cows
at post stage. Both plasma and milk antioxidative capacities
were enhanced in vit E injected cows at post and 7d post stages.
Plasma vit E level did not significantly correlated to plasma
CREA (creatinine) and TG (triglyceride) concentrations and CK
activity, but were positively correlated to plasma CHOL
(cholesterol) concentration and LDH activity (r2=0.49 and 0.52,
p<0.01, respectively). A negative correlation between RBC vit E
level and RBC GSH-Px activity was also found (r2=-0.48, p<0.05).
Milk serum SOD, GSH-Px and CAT activities were not correlated to
milk vit E level, but were positively correlated to milk SCC
(r2=0.76、0.78 and 0.69, p<0.01, respectively). A positive
correlation coefficient was demonstrated between milk SCC and
milk TP content (r2=0.57, p<0.01), but the correlation
coefficient between milk SCC and lactose level was negative
(r2=-0.61, p<0.01).Taken together, muscular injection with vit E
(3000 IU/day for 5 days), significantly increased plasma RBC and
milk vit E contents And antioxidative capacities in cows.
Injection treatment, however, may cause muscular damage and
increased stress indics ( etc. LDH and CK), but this treatment
did not affect plasma and milk components. In addition to higher
GSH-Px activity in RBC, no significant effect of vit E injection
was found on the other antioxidative enzyme activities in
plasma, RBC and milk serum. The antioxidative enzyme activities
in milk was consequently postulated to be derived from blood and
/or from milk SCC.Effects of short term vitamin E injection on
antioxidative capacity and lactation performance of Holstein cow
in hot seasonPin-Nan LeeSummary Vitamin E(vit E) is one of the
most important antioxidative micronutrients in animals. The
purpose of this study was to investigate the effects of short
term vit E injection(3000 IU/d, for 5 day)(i.m.) on the major
milk components, plasma metabolic contents, and milk, RBC and
plasma antioxidative enzyme activities and their vit E contents
of lactating Holstein cows. Twelve lactating cows were randomly
allocated into two groups: placebo injection as control and vit
E injection. Blood and milk samples were collected at day 0
(before vit E and placebo injection, pre-), day 5 (after
injection, post) and day 12 (one week after injection, 7d post).
The results suggested that the post vit E concentrations of
plasma, RBC and milk serum were significantly elevated by 3.3,
1.2 and 2.3 folds in the vit E treated cows as compared to those
of placebo-treated cows (p<0.05), levels then declined at 7d
post. Plasma LDH (lactate dehydrogenase) and CK (creatine
kinase) activities were significantly elevated after placebo and
vit E injection. No significant differences in plasma SOD
(superoxide dismutase), CAT (catalase) and GSH-Px activities
were found at all pre-, post- and 7d post stages between placebo
and vit E-treated cows, and neither did SOD and CAT activities
in RBC. RBC GSH-Px activity was decreasd significantly (p<0.05)
in vit E injection cows in comparison with placebo-treated cows
at post stage. Both plasma and milk antioxidative capacities
were enhanced in vit E injected cows at post and 7d post stages.
Plasma vit E level did not significantly correlated to plasma
CREA (creatinine) and TG (triglyceride) concentrations and CK
activity, but were positively correlated to plasma CHOL
(cholesterol) concentration and LDH activity (r2=0.49 and 0.52,
p<0.01, respectively). A negative correlation between RBC vit E
level and RBC GSH-Px activity was also found (r2=-0.48, p<0.05).
Milk serum SOD, GSH-Px and CAT activities were not correlated to
milk vit E level, but were positively correlated to milk SCC
(r2=0.76、0.78 and 0.69, p<0.01, respectively). A positive
correlation coefficient was demonstrated between milk SCC and
milk TP content (r2=0.57, p<0.01), but the correlation
coefficient between milk SCC and lactose level was negative
(r2=-0.61, p<0.01).Taken together, muscular injection with vit E
(3000 IU/day for 5 days), significantly increased plasma RBC and
milk vit E contents And antioxidative capacities in cows.
Injection treatment, however, may cause muscular damage and
increased stress indics ( etc. LDH and CK), but this treatment
did not affect plasma and milk components. In addition to higher
GSH-Px activity in RBC, no significant effect of vit E injection
was found on the other antioxidative enzyme activities in
plasma, RBC and milk serum. The antioxidative enzyme activities
in milk was consequently postulated to be derived from blood and
/or from milk SCC.
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