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研究生:張盈盈
研究生(外文):Chang, Ying-Ying
論文名稱:水楊酸、甲基茉莉香酸及BTH對番茄抗細菌性斑點病之影響
論文名稱(外文):Effect of salicylate, methyl jasmonate and benzothiadiazole on resistance of tomato plants to bacterial spot caused by Xanthomonas campestris pv. vesicatoria
指導教授:曾德賜
指導教授(外文):Dean Der-Syh Tzeng
學位類別:碩士
校院名稱:國立中興大學
系所名稱:植物病理學系
學門:農業科學學門
學類:植物保護學類
論文種類:學術論文
論文出版年:1998
畢業學年度:86
語文別:中文
論文頁數:65
中文關鍵詞:水楊酸番茄抗病
外文關鍵詞:salicylatemethyl jasmonatetomatoinduced resistancecatalase
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本研究旨在利用 Salicylate (SA)、 methyl jasmonate (MJ) 及
benzothiadiazole(BTH) 測試其在誘導番茄抗細菌性斑點病上之應用性並
探討其相關機制。以番茄農友 301栽培品種為試驗材料,初步篩選施用濃
度, 結果以 2.23 mM MJ、2.5 mM SA 與 0.28 mMBTH 效果較好, 三種
測試藥劑對 XCV 在生體外並無明顯抑制生長的作用,所以植株之抗病性
的提高,應由藥劑誘導抗病所形成的。 繼而以 SA、MJ 及 BTH 處理並於
處理後不同天數接種病原菌 (XCV),MJ 處理後上位葉在第二天即顯現抗
病性的提高,至第 8 天最為明顯,而下位葉則須四天後方顯現抗病性,
且持續到第 8 天; 以 SA 處理植株下位葉在第四天才開始顯現抗病性,
至第六天抗病表現達到最高且可持續到第 8 天,上位葉則在第六天才有
降低罹病度的現象,亦可持續至第八天。 而施用 BTH 者其上、下位葉由
第二天起其抗病能力即開始提升亦可持續到第 8 天。 施用藥劑之植株其
罹病組織內之菌量增殖速度較對照組慢,且接種十天後葉片組織之菌量約
只有對照組的十分之一。在研究中即有之結果顯示於施用 MJ
之番茄植株處理後其超氧岐化酵素 (superoxidedismutase,SOD) 及
多酚氧化酵素 (polyphenol oxidase, PPO) 活性較之對照組有提高之趨
勢, 過氧化氫脫氫酵素 (catalase, CAT) 之活性則較對照組為低,
而過氧化酵素(peroxidase, PO) 之活性則無明顯差異。 處理 BTH 者其
SOD, CAT, PO 與 PPO 之活性都較對照組為高, 而施用 SA 者其酵素活
性變化與對照組比較則較無明顯差異,而 H2O2含量則在第六天上升,施
用 MJ 後其植株酚類化合物及 H2O2 含量均有明顯提高之趨勢,此些證據
顯示施用 MJ、BTH 後植物組織中 SOD、CAT、PPO 活性變化以及酚類化合
物的含量增加等與抗病性的提高密切相關,而 SA 所誘導之抗病性其機制
仍需再一步探討
The main objective of this investigation were to explore
the potentialapplication of methyl jasmonate (MJ) ,
sodium salicylate (SA) , andbenzothiadiazole (BTH) in
the control of bacterial spots of tomato(Lycopersicon
esculentum cv. Known-You 301) caused by Xanthomonas
campestrispv. vesicatoria (XCV), and to elucidated the
mechanisms possibly involved. Apreliminary screening test
indicated that spray application of each of thethree tested
chemical individually at certain range of concentration
waseffective in reducing the infection of XCV on tomato
leaves. The effectiveconcentration appeared to be 2.23 mM
for MJ, 2.5 mM for SA and 0.28 mM forBTH. The observed disease
control efficacy was very likely due to the functionof enhanced
resistance of plant host since the test chemicals at
appliedconcentration were not bacterocidal against XCV in an in
vitro system. Afterthe chemical application, the
effectiveness was detected 2 days aftertreatment for both
MJ and BTH, and 6 days for SA. In tomato
plantsartificially inoculated with XCV, the propagation of
test bacteria in foliartissue was reduced by an order when a
pretreatment of these test chemicalenhancement of disease
resistance, changes of various redox enzymes andcertain
defensive chemicals were detected from test plant. The
application ofBTH greatly enhanced the activity of superoxide
dismutase (SOD), catalase(CAT), peroxidase (PO), and
polyphenol oxidase (PPO) in foliar tissue. Theeffectiveness
of BTH application seemed to be more prominent among
youngerfoliar tissue; and except that of PPO, the
effectiveness of enzyme activityenhancement appeared to be
strengthened or even dependent on the challengeinoculation of
XCV. Among MJ treated plants, the activation of SOD and PPO
wasalso detected, However, unlike that of BTH treatment, a
substantial reductionof catalase activity and an
accumulation of H2O2 and phenolic contentsappeared to remain
unchanged. Whereas in SA treated plants, most of mentionedredox
enzymes and defensive chemicals within the foliar tissue
remained at thesame level as that of water treated control,
although a transient increase ofPPO was detected when these
plants were challenge inoculated with XCV. Theprovided
evidence indicated that the disease resistance enhancement
providedby MJ or BTH application was due greatly in part to the
increased activity ofSOD, CAT, PO, and PPO and the accumulation
of defensive chemicals like H2O2 orphenolic compounds. The
reason how SA contributed to the observed resistanceenhancement
remained to be elucidated. The efficacy of disease control
ofthree tested chemicals appeared to be consistent, required
only low doses. Theenvironmental safety characteristic further
warrants their potential to becomeideal plant protectants for
the future.
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