臺灣博碩士論文加值系統

本研究採用孤挺花(Hippeastrum hybridum Hort.)三年生鱗球(球圍20
22 cm)，以米字形八分割切開，取由外向內3組雙鱗片為試驗材料，培
育於透明塑膠封口袋中。品種間比較，以''Apple Blossom''品種小鱗莖形
成率最好，''Best Seller''次之，''Red Lion''最差；不同成熟度的雙鱗片
以中層(PIIPIV)的小鱗莖形成率最高，外(PI)及內層(PV,PVI)較差。在
自然散射光或暗環境下小鱗莖形成率無顯著差異，但在自然散射光環境下
整體表現較好。在切法上以1/8 + 2及1/8 + 1切法較慣行之1/8切法可得
較多的小鱗莖，且小鱗莖重亦能達到種植標準，1/16切法整體表現最差。
雙鱗片基盤寬度愈大，小鱗莖形成數愈多，但與小鱗莖重量則相關性不顯
著。由單鱗片培育，會先形成擬球體再形成小鱗莖，而雙鱗片大部分直接
形成小鱗莖，亦有少數如單鱗片培育的情形。雙鱗片培育在有光條件下，
初期呼吸率較暗處理高，至第6天以後則較低，此與自然光環境下小鱗莖
形成發育有一致趨勢。雙鱗片培育期間全可溶性糖含量在第1及34週會
大幅度降低，第56週有一上升高峰出現而後又很快的降低，而澱粉含量
則於第14週呈緩慢下降，直到第4週之後時有一明顯降幅，此與小鱗莖
形成發育有相對應之關係。雙鱗片組織培養繁殖時，種球消毒以50℃90分
鐘或55℃45分鐘以上熱水處理可降低污染率至10 %以下。培養基蔗糖濃度
以50g/l處理可得到最多的小鱗莖；添加葡萄糖:果糖=15:15 g/l之處理小
鱗莖形成最多，單獨使用果糖30 g/l則表現最差。在光環境下培養基加活
性碳(3 g/l)可促進小鱗莖形成，且小鱗莖亦較重；添加BA雖可促進小鱗
莖形成，但對小鱗莖生長發育則沒有促進作用，濃度以不超過2.0 mg / l
為宜。

Three-year Hippeastrum bulbs of 20- to 22-cm circumference,
which were cut into eight parts evenly (1/8), were used to study
scaling. The outer one to three sets of twin-scale were treated
and were placed into a clear plastic bag for incubation. In
comparison among three cultivars, ''Apple Blossom'' rate of
bulblet formation was the best , then ''Best Seller'', and ''Red
Lion'' the least. The bulblet formation rate of the middle
layer twin-scales(PIIPIV) was the highest, then that of outer(
PI), and that of the inner(PVPVI) twin-scales was the least.
The number of bulblet obtained by using 1/8+1 or 1/8+2 cutting
method was larger than that of 1/8 conventional cutting method,
and that of 1/16 cutting method was the least. However, the
bulblet form both of 1/8 +1 or 1/8 +2 cutting methods could
reach the planting size(dia. 7.4 mm). As the basal plate width
of twin-scaling increased, the number of bulblet formation
increased, but no significant difference was found on weight of
plantlets.Protocorm-like bodies (PLBs) formed first before
developing to plantlets on single-scaling, but plantlets formed
directly and PLBs were found occasionally on twin-scaling, as
the same as single-scaling. Respiration of twin-scaling early
stage under light condition was higher than that under dark
period, but decreased down below than dark period 6 days after
cultivation. This trend was consistence with bulblet formation
under ambient light. Total soluble solid of twin-scale
decreased dramatically on the 1st and 3rd to 4th week and
decreased sharply after a rising peak on 5th to 6th week during
twin-scaling. Starch content decreased steadily on 1st to 4th
and decreased significantly after the 4th week while twin-
scaling. All these phenomena had relationship with bulblet
formation .Pasteurizing bulb by using 50C water for 90 minutes
or 55C for 45 minutes could decreased, the contamination rate
to 10% evenly lower while twin-scaling in vitro. The bulblet
number obtained was largest on sugar 50 g/L treatment. In
sucrose source treatment, bulblet number formed was largest in
glucose : fructose = 15 g/L : 15 g/L and was smallest in
fructose 30 g/L only. Bulblet formation was promoted while
activated carbon was added on the medium under light condition
and the bulb weight formed was heavier than the control set. BA
supplement enhanced bulblet formation, however, bulblet growth
and development was not promoted. Therefore, the BA
concentration is better to remain below 2.0 mg/L when twin-
scaling in vitro.