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研究生:湯惠媄
研究生(外文):Tang, Hui-Mei
論文名稱:固形材質在組培苗健化之研究
論文名稱(外文):Studies of the Solid Supporters on the Hardening of the
指導教授:黃敏展黃敏展引用關係
指導教授(外文):Huang Min-Chang
學位類別:碩士
校院名稱:國立中興大學
系所名稱:園藝學系
學門:農業科學學門
學類:園藝學類
論文種類:學術論文
論文出版年:1998
畢業學年度:86
語文別:中文
論文頁數:192
中文關鍵詞:組織培養健化液體培養固形材質
外文關鍵詞:tissue culturehardeningliquid culturesolid supporter
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本研究將洋桔梗、星辰花及非洲菊等三種不同生育習性的瓶苗培殖在插花
用海綿(oasis)、水耕用岩棉(rockwool)、穴盤用岩棉(mutiblock
rockwool)、人造海綿(sponge)、紙巾(paper towel)及不織布(non-woven
fabric)等固形材質,期能代替培養基固化時常使用的洋菜。同時改善瓶
苗長途運輸後之品質,簡化出瓶移植時的操作,並提高出瓶成活率試驗所
使用的基本培養液配方採用半量之MS配方含15g/l蔗糖。在培養洋桔梗時
,添加IBA 2 mg/l;培養星辰花時添加NAA 0.5 mg/l;培養非洲菊時添加
IBA 0.1 mg/l。經過5週培養後,在培養洋桔梗時以人造海綿、穴盤用岩
棉、不織布及紙巾支持物,培養星辰花時以不織布、人造海綿和紙巾為支
持物,培養非洲菊時,則用紙巾為洋菜支持物,瓶苗生長較培養於洋菜培
養基者為佳。使用固形材質時,需配合培養液量之水位高度。洋桔梗瓶苗
在使用人造海綿培養時,以材質吸飽水後沒有多餘養液水位為宜;使用穴
盤用岩棉培養時以0.5 cm養液水位為佳;使用不織布培養時則需1 cm之養
液水位;使用紙巾培養時以1.5 cm養液水位較好。而星辰花瓶苗使用紙巾
培養時以沒有多餘養液水位為佳;使用人造海綿培養時以1 cm養液水位較
好;使用不織布培養時1.5 cm養液水位為最適。但洋桔梗和星辰花瓶苗移
植前培養期間大體上生長雖好,卻仍有極少部分產生水化現象。但上述固
形材質若配合使用蓋上開孔後另加貼超微細過濾膜的培養容器,在使用人
造海棉和紙巾培養洋桔梗和星辰花瓶苗時,則有健化瓶苗及增進生長之作
用。另外,為建立瓶苗有自營生長能力,進行高光和低糖環境培養。結果
不論使用何種固形材質,在高光下,隨著培養液中糖濃度的降低,瓶苗生
長發育表現更好,其中以培養無糖培養基的瓶苗生長最佳,出瓶後成活率
最高。且使用無糖培養液時,只要使用的固形材質清潔,培養液也可以不
經滅菌處理即使用。在明期時每隔8小時添加2公升量的CO2,使生長箱中
CO2濃度提昇至大約1700 ppm,可促進瓶苗建立自營能力。如洋桔梗和星
辰花瓶苗添加CO2培養,在外觀形態品質均優於未添加CO2處理者,其他如
葉片氣孔分布密度較低,形狀變為較大、長橢圓形、且具開閉功能。
The explants of the different habitual crops such as Lisianthus,
Statice, and Gerbera were cultured on solid supporters, such as
oasis, rockwool, multiblock rockwool, sponge, paper towel, or
non-woven fabrics solid supporters to substitute the solidified
media by agar, respectively, in order to improve the quality
after long term transportation, to simplify the operation of
transplanting, and to increase the survival rate ex vitro. The
basal MS medium contained sugar 15 g/l, and IBA 2mg/l, NAA
0.5mg/l, or IBA 0.5mg/l when cultured prairie gentian , statice
or gerbera, respectively. After in vitro culture for 5 weeks,
when the supporters of sponge, multiblock rockwool , non-woven
fabric or paper towel were used for prairie gentian, non-woven
fabric and paper towel was used for statice, as well as paper
towel was used for gerbera, the growth of explants on those
solid supporters were better than those cultured on agar media.
The volume of liquid medium was response to the kind of
supporter used. When prairie gentian explants were planted on
sponge, multiblock rockwool, non-woven fabric, or paper towel,
the most suitable liquid level for culture was at 0cm (just
saturation), 0.5cm, 1cm, or 1.5cm, respectively. When statice
planted on paper towel, sponge, or non-woven fabric the best
liquid level was at 0cm , 1.0cm, and 1.5cm, respectively.
Sometimes there were a few of hydrosis of prairie gentian and
statice explants, when cultured on liquid media. However, the
container with permeable filters enhanced hardening and growth
of prairie gentian and statice explants cultivated on sponge and
paper towel.In addition, to establish the autophotosynthesis of
explants, culture was conducted under low sugar medium in the
medium and high photosynthetic photon flux density environment.
Plantlets growth was the better when plantlets were cultured on
the lower sugar concentration. It means when plantlets were
grew on medium without sugar, the survival rate ex vitro was the
highest. Moreover, liquid medium did not be sterilized if the
solid supporters were clean.During light period, enriching 2
liter CO2 in growth chamber every 8 hours to increase CO2
concentration to 1700 ppm promoted the autophotosynthesis
ability of plantlets. It means the plantlets of prairie gentian
and statice had better quality in appearance and had lower
density but larger ellipsoid and functional stomata.
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