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研究生:陳振義
研究生(外文):Chen, Jenn-Yih
論文名稱:紫背與綠背一點植物性狀與組織培養之研究
論文名稱(外文):Studies on Botanical Characters and Tissue Culture of Nervilia purpurea and N.aragoana
指導教授:葉茂生葉茂生引用關係
指導教授(外文):Mau-Shing Yeh
學位類別:碩士
校院名稱:國立中興大學
系所名稱:農藝學研究所
學門:教育學門
學類:專業科目教育學類
論文種類:學術論文
論文出版年:1998
畢業學年度:86
語文別:中文
論文頁數:1
中文關鍵詞:一點植物性狀組織培養
外文關鍵詞:Nervilia purpureaN.aragoanabotanicalcharactertissue culture
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本研究利用屏東縣恆春所產之紫背與綠背一點為材料,移植於中興大學
農藝系溫室中,調查其植株性狀並將其生長情形記錄成生活史。利用球莖
分別種植於A(砂+泥炭土,1:1)、B(土壤+泥炭土,1:1)兩種不同栽培介質
,比較其萌芽率,以選擇適合生長的土壤介質。並利用植株之走莖、球莖
、葉柄及葉片等四部分,以組織培養方式來探討紫背與綠背一點其植株
之再生及適合繁殖之條件,以建立其快速繁殖技術,作為大量繁殖之用,
其結果如下:
1.紫背與綠背一點之球莖,種植於A、B兩種栽培介質中,半年後調查其
萌芽率,以A種栽培介質效果最佳,紫背一點之球莖萌芽率達100﹪,而
綠背一點之球莖萌芽率達93.3﹪,且對球莖增大效果亦佳。
2.紫背與綠背一點不同部位培養60天後之結果,紫背一點以走莖和球
莖部位有反應,走莖頂端部位產生白色毛狀物後伸出走莖,其形成率光暗
分別有22﹪及26.7﹪。綠背一點只有走莖部位有反應,形成綠色芽體後
形成走莖。以添加1mg/l BA及0.2mg/l NAA之MS培養基(代號BN1-0.2)誘導
走莖之效果較佳。
3. 紫背一點繁殖之走莖,在添加0.5mg/l BA及1mg/l NAA之MS培養基(
代號BN0.5-1),有較佳的效果、經90天後培養,瓶底可形成球莖,而且將
這些球莖種植後,會直接長成植株。
4. 綠背一點繁殖走莖試驗,以添加0.5mg/l BA及1mg/l NAA(代號
BN0.5-1)培養基經90天培養後,可繁殖較長的走莖,繁殖過程中並沒有球
莖的產生。
5.綠背一點誘導之走莖,以MS培養基添加1.5g/l peptone及1.5g/l活性
碳可誘導出較多植株(26株),之後再形成小球莖。
綜合以上之試驗,紫背與綠背一點利用球莖繁殖,以A(砂+泥碳土
,1:1)栽培介質為最佳生長的土壤性質,因此,在栽培方面應選擇土質疏
鬆富有機質之土壤條件,有助於其生長。其次,以組織培養方式繁殖紫背
與綠背一點之走莖部位,紫背一點在添加0.5mg/lBA及1mg/lNAA之MS(
代號BN0.5-1)之培養基中平均可誘導出20個球莖,時間為5個月,而綠背
一點在走莖誘導再生植株,以MS培養基添加1.5g/l peptone及1.5g/l活
性碳最佳,平均每瓶所繁殖之走莖可誘導26株小苗,時間為6∼8個月。
Studies on Botanical Characters and Tissue Culture of
Nervilia purpurea and N. aragoana.

Summary

The purpose of this study was to investigate the agronomic
characters, and to find out a suitable cultural medium for N.
purpurea and N. aragoana ,which origined from Pyng Dong .
Further, try to explore the best condition for plantlet
regeneration through tissure culture, in order to establish a
quickly propagation technique.
The results were shown as following:
1. Culture medium A(sand+peat ,1:1) was better than medium B(
loam+peat ,1:1) for corm propagation. At A medium, the
emergence rate was 100% for N. purpurea and 93.3% for N.
aragoana after 6 months corm sowing.
2. Sixty days culture from different parts of plant , trichome
was appeared on the top of rhizome and stretch rhizome for N.
purpurea, green shoot appeared and then stretched rhizome for N.
aragoana . The best medium for rhizome induction was MS
supplemented with 1mg/g BA and 0.2mg/l NAA.
3. The best medium of rhizome propagation for N. purpurea was
MS supplemented with 0.5mg/lBA and 1mg/lNAA(Code BN0.5-1).After
90 days culture, corm could be induced and become plantlet
after sowing.
4. The best medium of rhizome propagation for N.aragoana was
MS supplemented with 0.5mg/lBA and 1mg/lNAA(Code BN0.5-1).
After 90 days culture, longer rhizome could be obtained without
corm induction.
5. Plantlet could be induced from rhizome of N.aragoana
through MS supplemented with 1.5g/lpeptone and 1.5g/l activated
charcoal.
From the above results, It was suggested that medium A(sand
+peat,1:1) was the best medium for corm propagation and loam
soil with rich organism matter for plant growth. In tissue
aspect, corm could be induced form rhizome of N.purpurea at MS
supplemented with 0.5mg/l BA and 1mg/l NAA, and plantlet could
be induced from rhizome of N.aragoana at MS supplemented with
1.5g/lpeptone and 1.5g/l activated charcoal medium. The former
required 5 months, and latter required 6~8 months.
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