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Studies on Botanical Characters and Tissue Culture of
Nervilia purpurea and N. aragoana.
Summary
The purpose of this study was to investigate the agronomic
characters, and to find out a suitable cultural medium for N.
purpurea and N. aragoana ,which origined from Pyng Dong .
Further, try to explore the best condition for plantlet
regeneration through tissure culture, in order to establish a
quickly propagation technique.
The results were shown as following:
1. Culture medium A(sand＋peat ,1:1) was better than medium B(
loam＋peat ,1:1) for corm propagation. At A medium, the
emergence rate was 100% for N. purpurea and 93.3% for N.
aragoana after 6 months corm sowing.
2. Sixty days culture from different parts of plant , trichome
was appeared on the top of rhizome and stretch rhizome for N.
purpurea, green shoot appeared and then stretched rhizome for N.
aragoana . The best medium for rhizome induction was MS
supplemented with 1mg/g BA and 0.2mg/l NAA.
3. The best medium of rhizome propagation for N. purpurea was
MS supplemented with 0.5mg/lBA and 1mg/lNAA(Code BN0.5-1).After
90 days culture, corm could be induced and become plantlet
after sowing.
4. The best medium of rhizome propagation for N.aragoana was
MS supplemented with 0.5mg/lBA and 1mg/lNAA(Code BN0.5-1).
After 90 days culture, longer rhizome could be obtained without
corm induction.
5. Plantlet could be induced from rhizome of N.aragoana
through MS supplemented with 1.5g/lpeptone and 1.5g/l activated
charcoal.
From the above results, It was suggested that medium A(sand
＋peat,1:1) was the best medium for corm propagation and loam
soil with rich organism matter for plant growth. In tissue
aspect, corm could be induced form rhizome of N.purpurea at MS
supplemented with 0.5mg/l BA and 1mg/l NAA, and plantlet could
be induced from rhizome of N.aragoana at MS supplemented with
1.5g/lpeptone and 1.5g/l activated charcoal medium. The former
required 5 months, and latter required 6~8 months.
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