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Studies on Botanical Characters and Tissue Culture of Nervilia purpurea and N. aragoana.
Summary
The purpose of this study was to investigate the agronomic characters, and to find out a suitable cultural medium for N. purpurea and N. aragoana ,which origined from Pyng Dong . Further, try to explore the best condition for plantlet regeneration through tissure culture, in order to establish a quickly propagation technique. The results were shown as following: 1. Culture medium A(sand+peat ,1:1) was better than medium B( loam+peat ,1:1) for corm propagation. At A medium, the emergence rate was 100% for N. purpurea and 93.3% for N. aragoana after 6 months corm sowing. 2. Sixty days culture from different parts of plant , trichome was appeared on the top of rhizome and stretch rhizome for N. purpurea, green shoot appeared and then stretched rhizome for N. aragoana . The best medium for rhizome induction was MS supplemented with 1mg/g BA and 0.2mg/l NAA. 3. The best medium of rhizome propagation for N. purpurea was MS supplemented with 0.5mg/lBA and 1mg/lNAA(Code BN0.5-1).After 90 days culture, corm could be induced and become plantlet after sowing. 4. The best medium of rhizome propagation for N.aragoana was MS supplemented with 0.5mg/lBA and 1mg/lNAA(Code BN0.5-1). After 90 days culture, longer rhizome could be obtained without corm induction. 5. Plantlet could be induced from rhizome of N.aragoana through MS supplemented with 1.5g/lpeptone and 1.5g/l activated charcoal. From the above results, It was suggested that medium A(sand +peat,1:1) was the best medium for corm propagation and loam soil with rich organism matter for plant growth. In tissue aspect, corm could be induced form rhizome of N.purpurea at MS supplemented with 0.5mg/l BA and 1mg/l NAA, and plantlet could be induced from rhizome of N.aragoana at MS supplemented with 1.5g/lpeptone and 1.5g/l activated charcoal medium. The former required 5 months, and latter required 6~8 months.
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