本研究使用四個梗型品種及二個秈型品種為雜交親本, 交配成四組秈、梗稻雜交組合做為
試驗材料, 並以單粒後裔法(SSD), 田間栽培混合法(BF)及溫室栽培混合法(BG)選育秈、梗
雜交後代, 以了解族群基因頻度的變化。試驗中調查雜交族群之F2至F7世代的酸性磷酸脢
Acp-1、過氧化脢Pox-1及苯醇反應Ph等三基因座之基因頻度變化, 茲將結果分述如下:
1. 在Acp-1基因座上, 四個雜交組合的三種選育方法中, 雜交後裔族群之Acp-1-4基因頻度
均較高, 有偏向秈型親本的分離情形。
2. Java 14 * CNS 6雜交組合的Pox-1基因座基因之分離比, 在三選育方法中幾乎完全符合
孟德爾遺傳, 即與秈梗分化無關的基因在秈梗雜交後裔族群中不發生偏離現象。
3. 在Ph基因座, 四個雜交組合的三種選育方法中, 雜交後裔族群之Ph+表現型頻度均較高,
有偏向秈型親本的分離情形, 與Acp-1-4基因型的表現一致。
4. 本試驗之結果顯示, 單粒後裔法較具有族群變異性的潛力, 溫室栽培混合法之基因頻度
變化較具穩定性, 但此二種與世代促進有關之選育法, 其雜種後裔族群內基因頻度之變化
與田間栽培混合法並無顯著差異。

To detect the changes of gene frequency of Indica-Japonica rice
hybrid progenies
using single seed descent (SSD) method, bulk method in the
field (BF) and in the
greenhouse (BG), four rice hybrids were crossed from four
Japonica types and two
Indica types. From F2 through F7 generations, the gene
frequencies od acid phosphatase
(Acp-1), peroxidase (Pox-1) and phenol reaction (Ph) were
investigated. The results
were summarized as follos:
1. All four hybrids derived from SSD, BF and BG methods had higher frequency in
Acp-1-4. In other words, the hybrid progenies were skewed in
favor of Indica parents
in Acp-1 locus.
2. The segregation ratio of Pox-1Nul and Pox-12A derived from
Java 14*CNS 6 in three
selection populations were in accordance with Mendelian ratio
theory. Consequently,
the gene which did not belong to Indica or Japonica type were not skewed.
3. All four hybrids derived from SSD, BF and BG methods had
higher frequency in Ph+
form. It methoded that hybrid progenies were skewed in favor of
Japonica parents in
Ph locus, therefore, the performance distribution of Ph was
equaled to that of Acp-1.
4. The results showed that the SSD population had the ability
of recovering population
variation. But these two populations that improved acclerated
generation cycle and BF
population were no differences in gene frequencies variation.