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研究生:劉必揚
研究生(外文):Liu, Bia-Yang
論文名稱:本省豬場三段式廢水處理之細菌學檢查
論文名稱(外文):Bacteriological examination of the three-step waste-water treatment system in swine farms of Taiwan
指導教授:張登欽張登欽引用關係
指導教授(外文):Chung Ting-Ching
學位類別:碩士
校院名稱:國立中興大學
系所名稱:獸醫學系
學門:獸醫學門
學類:獸醫學類
論文種類:學術論文
論文出版年:1998
畢業學年度:86
語文別:中文
論文頁數:91
中文關鍵詞:三段式廢水處理細菌
外文關鍵詞:three-step waste-water treatmentbacteria
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摘要自民國86年1月起到10月止,於本省中部地區三個豬場,分成冬、夏
兩季,每次各3個月,進行廢水經三階段處理後,殘存的總生菌數、大腸
桿菌群數及重要病原菌(沙氏桿菌與豬丹毒桿菌)的調查;並分析豬場廢
水中抑菌物質;於實驗室中並將將沙氏桿菌與豬丹毒桿菌,接種至實驗室
的廢水處理模型,分別於不同的溫度條件下觀察兩者的消長情形。實驗的
結果顯示,各豬場的總生菌數與大腸桿菌群數,經廢水處理後呈現顯著減
少的情形;在冬、夏季間,除夏季厭氣處理後的總生菌數高於冬季外,其
餘則無顯著地差異;而A豬場在固液分離後廢水中的總生菌數與大腸桿菌
群數,皆顯著地高於其他兩豬場。大部分的廢水中都無法分離到沙氏桿菌
與豬丹毒桿菌,僅B場的曝氣處理後,分離到4株沙氏桿菌(Salmonella
choleraesuis),經過抗藥性的分析,所有沙氏桿菌皆對一種以上的抗生
素產生抗藥性,對Ampicilline、Chloramphenicol、Kanamycin及
Tetracycline則有很高的MIC值。以Cylinder plate assay可以檢測到豬
場各階段廢水的上清液,都會在各試驗平板上產生抑制圈,惟試驗中無法
明確指出抑菌物質為何。廢水處理模型在環境溫度為35℃、30℃、16℃的
條件下,接種沙氏桿菌後,於厭氣醱酵槽中分別可殘存16、27、55天,經
過厭氣處理後在曝氣槽中分別可殘存12、18、21天;而豬丹毒菌無法自廢
水中分離,此與現場病原菌的分離結果相同。綜合上述諸點,可知目前本
省豬場三段式廢水處理系統,在正常的操作下,並無法完全去除病原菌(
沙氏桿菌),尤其當豬場有爆發疫情時,大量病原菌會隨豬糞尿進入廢水
處理設施中,必須特別注意排放水中病原菌的殘留問題。
AbstractA bacteriological examination, including total count,
coliform-group count, and pathogens detection (i.e., Salmonella
spp. and Erysipelothrix rhusiopathiae), was conducted from Jan.
1997 till Oct. 1997 as to evaluate the efficiency of the three-
step waste-water-treatment systems established by three pig
farms located at the middle area of Taiwan. Also included in
this work are the detection of antimicrobial substance in the
samples of waste water and the in vitro analysis of the survival
of Salmonella choleraesuis and Erysipelothrix rhusiopathiae in
two simulators of the three-step waste-water-treatment system.
Analysis of the bacterial counting as well as the pathogen
isolations revealed that: (1) significant declines of both of
total counts and coliform-group counts were observed in each
consecutive stage of waste-water treatment system among all
three pig farms examined, (2) no significant difference of
bacterial numbers between summer and winter seasons were
observed, except that of total count numbers after the anaerobic
chamber stage which actually showed a significant difference
between the two seasons, (3) four strains of Sal. choleraesuis
were, respectively, isolated from four waste water samples
collected after the aerobic treatment stage in farm B, (4)
multiple antibiotic resistance characteristics, especially with
respect to Ampicilline, Chloramphenicol, Kanamycin, and
Tetracycline which showed a very high minimum inhibitory
concentration (MIC) value, were detected in all four strains of
Sal. Choleraesuis, (5) all field-samples showed distinguishable
inhibitory zones in Cylinder-plate-assay (i.e., a bio-assay for
the detection for the antimicrobial substance by the appearance
of certain size of inhibitory zone), albeit the qualtative
analysis as to exactly identify what kind of antimicrobial
substance existing in the sample were infeasible, (6) Sal.
choleraesuis under the circumstances of 35℃, 30℃, and 16℃in
the simulator study, were able to survive for a period of 16,
18, and 55 days respectively after the anaerobic treatment,
whereas a period of 12, 18, and 21 days of survival were
detected after the aerobic treatment, (7) Erysipelothrix
rhusiopathiae was not found in all the samples including the
field and the in vitro studies.In conclusions, the three-step
waste-water-treatment system used in Taiwan pig farm seems not
powerful enough as to remove pathogen such as Sal. choleraesuis,
especially at the epidemic outbreak which incur a large quantity
of pathogenic microbes excreted into the waste water.
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