本研究中第一個目標是以薄層層析法,分析Sulfamethazine(SMT),
Sulfadimethoxine(SDM),Sulfamonomethoxine(SMM),Sulfaquinoxaline(
SQX)及Sulfathiazole(STZ)等五種磺胺劑混合標準液,在展開後,呈現的螢
光帶,能區分這五種磺胺劑,實驗結果顯示薄層層析板以30 C水浴之展開液
[氯仿-正丁醇(80:20)],展開10公分,再以室溫之展開液,展開17 公
分,則可達成良好的分離效果.其最低檢測值為1~3 ug/ml. 第二個研究
目標是以高效液相層析(HPLC)法,同時測定與分離屠宰豬隻尿液中五種磺
胺劑(SMT,SDM,SMM,SQX與STZ). 高效液相層析法以分離管(Cosmosil
5C-18-AR)配合移動相[pH 3.6之醋酸緩衝液-Acetonitrile(85:15)],
以267 nm波長之紫外光檢測器,可精確地分析上述五種磺胺劑. 屠宰豬
尿液以稀硫酸液與氯仿二次萃取,以無水硫酸鈉脫水,經減壓濃縮至乾.反
相淨化方面,以pH 5.5醋酸液定容後,置於C-18管柱中,以蒸餾水洗淨,以甲
醇洗出.正相淨化方面,以甲醇液定容,置於氧化鋁淨化管中,以甲醇液洗
淨,以甲醇-醋酸液洗出.再經減壓濃縮至乾,以移動相定容,作為檢液,其
反,正相回收率分別為29.3~85.5 %與63.5~77.4 %,其最低檢測值為0.1
ug/mL. 酵素連接免疫分析法之套組檢測Sulfamethazine與
Sulfadimethoxine,二者之最低檢測值為0.001 ug/mL,對其它四種磺胺劑
之交叉反應性為0.1~2.9.

The first purpose of this investigation was developing a
thin-layerchromatograghic (TLC) method for applying mixed
standard solution contain-ing sulfamethazine (SMT),
sulfadimethoxine (SDM), sulfamonomethoxine (SMM),
sulfaquinoxaline (SQX) and sulfathiazole (STZ).Plate was
developed 10 cm in chloroform-n-BuOH(80:20) at 30 C water bath,
then the same plate was developed 17 cm at room temperature,
which were obtained the good seperationeffects. The limit
detection concentration of TLC method was 1~3 ug/mL. The second
purpose of this investigation was to develop a high- perform-
ance liquid chromatographic (HPLC) method for the simultaneous
determinationof SMT, SDM, SMM, SQX and STZ in the slaughtered-
pig urine. The separation of HPLC method was carried out on C18
column (Cosmosil 5C-18-AR column;4.6 x 150 mm I.D.)with acetic
acid buffer solution (pH 3.6)-acetonitrile (85:15) as the mobile
phase at a flow rate of 1.0 mL/min. Thechemicals were detected
by UV detector which the wavelength was set at 267nm. The
samples were prepared by extraction with 1 N sulphuric acid and
chloro- form twice, then were treated with solid phase
dispersion for cleanup. The recovery rates of the chemicals from
the slaughtered-pig urine were fortifiedat the level of 0.02~0.5
ug/mL which were 29.3~85.5 % and 63.5~77.4 % by reversal and
normal cleanup. The last purpose of this investigation was to
research the quality of ELISAmethod. The limit detection
concentration of ELISA method was 0.001 ug/mL forSMT and SDM,
The cross-reactivity of the other four sulfonamids on ELISA
methodwere 0.1~2.9 %.