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研究生:沈啟智
研究生(外文):SHEN, Chi-chih
論文名稱:在攪拌式發酵槽中置入不織布對於Acinetobacterradioresistens發酵生產耐鹼性脂肪酵素的影響
論文名稱(外文):The effect of equipping a non-woven fabrics in the fermentor on the production of Acinetobactor radioresistens lipase by batch fermentation.
指導教授:陳特良
指導教授(外文):Chen Teh-liang
學位類別:碩士
校院名稱:國立成功大學
系所名稱:化學工程學系
學門:工程學門
學類:化學工程學類
論文種類:學術論文
論文出版年:1998
畢業學年度:86
語文別:中文
論文頁數:69
中文關鍵詞:不織布脂肪酵素
外文關鍵詞:non-woven fabricslipase
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中文摘要
Acinetobacter radioresistens為一株自廢水汙泥中分離出來的革蘭式陰
性菌,其所生產之胞外脂肪酵素(triacylglycerol acylhydrolase, EC
3.1.1.3)在高鹼性的條件下具有相當高的活性。本研究著重於以批式醱酵
方式,探討將不織布置入2升醱酵槽中對A. radioresistens生產脂肪酵素
的影響。所採用的不織布組成原料為耐隆6(nylon 6),其表面淋澆有親油
性的壓克力樹脂。
在以正十六烷為碳源的自由細胞批次醱酵槽實驗中,吾人發現,菌體於75
小時才進入對數生長期,而且在102小時脂肪酵素活性可達最高,其值
為23 U/mL。然而,在不織布系統中,菌體進入對數生長期的時間較早(60
小時),而且其菌體濃度較高,在167小時脂肪酵素活性則增加到53 U/mL
。若在培養基中添加0.1%橄欖油,其自由細胞系統的醱酵在21小時脂肪酵
素活性只有16 U/mL,並且於20小時開始產生大量的泡沫;相反地,在使
用不織布的系統中,脂肪酵素活性可在84小時達到71 U/mL。此外,在後
者的系統中發現並沒有過量的泡沫產生。吾人亦得到一個結果,在醱酵槽
中置入仍布與自由細胞系統中添加阿拉伯膠的功能類似,皆可增加正十六
烷的分散性。 基於上述的發現
,吾人可將不織布在以A. radioresistens的醱酵行為中所扮演的角色做
以下的描述:(1)它幫助正十六烷分散成非常小的油滴,而有利於菌體生
長以及脂肪酵素的生產;(2)它可吸附在醱酵過程中所產生的界面活性劑
,進而抑制泡沫的生成。
ABSTRACT
Acinetobacter radioresistens, a Gram-negative bacterium isolated
from the sludge of waste water, produces an extracellular lipase
(triacylglycerol acylhydrolase, EC 3.1.1.3), which has a high
activity in alkaline pHs. This study intended to examine the
effect of equipping a non-woven fabrics in a 2-L tank fermentor
on the production of A. radioresistens lipase by batch
fermentation.The non-woven fabrics was made from nylon 6 coated
with a hydrophobic acrylic resin.
It was found that, in the free-cell fermentation with n-
hexadecane as the carbon source, the cells entered the
exponential growth phase at 75 h, and the maximum lipase yield
was 23 U/mL at 102 h. In the non-woven-fabrics system, however,
the cells entered the exponential growth phase earlier (at 60
h), the cell concentration attained was higher, and the lipase
yield increased to 53 U/mL at 167 h. When the medium was
supplemented with 0.1% olive oil, the lipase yield obtained in
the free-cell system was 16U/mL at 21 h, owing to excessive
foaming occurred at 20 h; in contrast, the lipase yield was 71
U/mL at 84 h if using the non-woven-fabrics system. In
addition, excessive foaming was not observed in the latter
system. The results obtained with the non-woven-fabrics system
were found comparable to that of the free-cell system where gum
arabic was added to emulsify n-hexadecane.
Based on these findings, the roles of the non-woven fabrics in
the fermentation with A. radioresistens can be pictured: (1) it
helps the dispersion of n-hexadecane to very fine oil droplets,
and thus favors cell growth and lipase production; and (2) it
can adsorb surface active agents produced during the
fermentation, and thus suppress the foaming.
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