Acinetobacter radioresistens可生產能夠水解三酸甘油脂的嗜鹼性脂
肪酵素。已知脂肪酵素的生產是由橄欖油所誘導﹐然而當橄欖的水解產
物(油酸)在培養基中累積時﹐卻會對脂肪酵素的生產加以抑制。以脈衝式
添加橄欖油的半批次培養可以在這兩種相對的效應間取得平衡﹐使得脂肪
酵素活性達到56 U/ml﹐隨後酵素活性即因氮源的缺乏而不再提升。如果
採取連續添加橄欖油與高濃度LB培養基的方式進行半批次培養﹐可使脂肪
酵素活性高達110 U/ml。
Acinetobacter radioresistens produces an alkalophilic lipase
which is capable of enzymatic hydrolysis of triacylglycerols.
Lipase synthesis was shown to be induced by olive oil and
repressed when the products of olive oil hydrolysis (oleic
acids) accumulate in the medium. A balance between these two
antagonistic effects was obtained in a fed-batch cultivation
with pulsed feeding of olive oil. Lipase production ceased when
the activity reached approximately 56U/ml due to a nitrogen-
source shortage. A continuous supply of olive oil and
concentrated LB medium allowed the lipase production to continue
to 110U/ml.
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