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研究生:馬翊峻
研究生(外文):Ma, Yi-Jun
論文名稱:聚三羥基丁酸合成菌之篩選
論文名稱(外文):Screening of poly-B-hydroxybutyrate producing bacteria
指導教授:陳虹樺陳虹樺引用關係
指導教授(外文):Chen, Hong-Hua
學位類別:碩士
校院名稱:國立成功大學
系所名稱:生物學研究所
學門:生命科學學門
學類:生物學類
論文種類:學術論文
論文出版年:1998
畢業學年度:86
語文別:中文
中文關鍵詞:聚三羥基丁酸本土細菌菌株Poly-β-hydroxybutyrate (PHB)Poly-β-hydroxybutyrate (PHB)聚三氫氧基丁酸Poly-β-hydroxybutyrate (PHB)Poly-β-hydroxybutyrate (PHB)生物學
外文關鍵詞:Corynebacterium sp. CBIndigenous bacteriaPHBBIOLOGY
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Poly-b-hydroxybutyrate (PHB) is an intracellular carbon and
energy storagematerial accumulated in many bacteria. Its
biodegradable properties can be usedin various ways similsr to
plastics derived from conventional petrochemicals.To establish
PHB-producing and biosynthesis gene screening systems,
Alcaligenes eutrophus H16 was used as a reference bacteria.
First, high C/N ratio (C/N=76)and molasses (5%) media were
selected for PHB production. Under thesis conditions,PHB content
was accumulated to 44% of bacteria dry weight. PHB biosynthesis
genesfrom Alcaligenes eutrophus H16 were cloned by colony
hybridization probed with 780 bp conserved phbC fragment. In
order to screen the PHB-producing indigenousbacteria, PCR
followed by Southern blotting analysis was carried out. Result
showed that a 780 bp PCR amplified fragment was detected in
three (Alcaligenesfaecalis NE and unidentified strains T2, NF)
of five indigenous bacteria, including;Corynebacterium sp. CB
showed a smear from 780 bp to 300 bp by touchdown PCR.Production
of PHB in both Corynebacterium sp. CB and unidentified strain T2
wereconfirmed by extraction of PHB and transmission electro-
microscopy. The PHB contents in Corynebacterium sp. CB and
unidentified strain T2 were 0.1 g/l and 0.08 g/l respectively.
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