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研究生:蘇文正
研究生(外文):Su, Wen-Zheng
論文名稱:Identificationandcharacterizationofthepromoterregionofmurineprothymosinα
指導教授:蕭璦莉
指導教授(外文):Xiao, Li-Qi
學位類別:碩士
校院名稱:國立成功大學
系所名稱:微生物學及免疫學研究所
學門:生命科學學門
學類:微生物學類
論文種類:學術論文
畢業學年度:86
語文別:中文
論文頁數:65
中文關鍵詞:前胸腺激素綠色螢光蛋白轉錄因子細胞週期啟動子微生物學免疫學
外文關鍵詞:prothymesin aEGFPtranscriptional factorcell cyclepromoterMICROBIOLOGYIMMUNOLOGY
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Prothymosin a (ProT a) is an acidic nuclear protein that is a member of thymic hormones; it can act as an immunomodulator and has an intracellular function associated with cell proliferation. It is expressed at high levels in a wide variety of cell types and may function in a c-myc associated pathway. Although the exact function of ProTa remains unknown, its highly evolutionary conservation and its wide tissue distribution suggest that it may play an essential role in the cell. In order to study the regulatory mechanisms of ProTa expression, we isolated a series of murine DNA fragments upstream the coding sequence of the murine ProTa gene. These fragments were linked to a reporter gene, the enhanced green fluorescent protein (EGFP), to construct a series of reporter plasmids which were used to transfect into NIH 3T3 cells. The promoter activity of ProTa can be identified by analyzing the expression of EGFP activity. The EGFP, driven by the CMV promoter, was used as a positive control. The positive transformants showing green light of EGFP could be observed under fluorescent microscope. After G418 selection, the plasmid-transfected cell lines were obtained and the EGFP expression was measured using flow cytometry. In this study, the analysis of a series of the synchronized cell lines reveals the ProT a promoter is regulated by transcription factors and is independent on the cell cycle.

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