(54.236.62.49) 您好!臺灣時間:2021/03/06 11:30
字體大小: 字級放大   字級縮小   預設字形  
回查詢結果

詳目顯示:::

我願授權國圖
: 
twitterline
研究生:鄭昇沛
研究生(外文):Cheng, Shen-Pei
論文名稱:壓電晶體免疫感測器於醫療檢驗之應用
論文名稱(外文):The appliciation of piezoelectric crystal immunosensor in clinical diagnosis
指導教授:張正, 詹爾昌
指導教授(外文):C. Allen Chang, Err-Cheng Chan
學位類別:碩士
校院名稱:國立交通大學
系所名稱:生物科技研究所
學門:生命科學學門
學類:生物科技學類
論文種類:學術論文
論文出版年:1998
畢業學年度:86
語文別:中文
論文頁數:87
中文關鍵詞:壓電晶體
外文關鍵詞:piezoelectric crystal
相關次數:
  • 被引用被引用:6
  • 點閱點閱:213
  • 評分評分:系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔系統版面圖檔
  • 下載下載:0
  • 收藏至我的研究室書目清單書目收藏:0
壓電晶體生物免疫感測器,其具有高靈敏性、高選擇性、即時分析、
成本低及快速的優點。在臨床檢驗上,極需利用這樣的特性以加速檢體的
測試,而給予醫師一個迅速確實的結果報告,達到患者病情治療的效果。
本研究將壓電晶體感測器結合免疫反應,利用polyethyleneimine及
glutaraldehyde固定抗體或抗原於壓電晶體上。而此法所製備之免疫探針
,應用於以下三種檢測:(一)、幽門螺旋桿菌抗體的測定 萃取幽門
螺旋桿菌蛋白固定於壓電晶片,來偵測感染宿主血清中抗幽門螺旋桿菌抗
體的存在。實驗結果顯示於血清樣本高稀釋條件下,稀釋倍數與頻率衰減
呈線性相關。在此稀釋倍數(70-500倍)範圍內,不但可去除因溶液黏度性
質改變而致頻率變化的變因外,也降低了正常血清樣本非專一性結合的干
擾。此外增加注入樣本量至250μL,樣本於稀釋70倍下,感染血清與正常
血清其衰減頻率差距達100Hz。當測量完成時,晶片可以利用1M NaOH脫覆
晶體上的抗體,使晶片重複使用達10次以上。(二)、金黃色葡萄球菌的
鑑別 本實驗以Anti-protein A antibody,IgG或血漿固定於晶片做為
測量金黃色葡萄球菌探針,其中以Anti-protein A antibody探針效果最
好,於2.14 x 105/mL菌量下,即可因頻率衰減差20Hz而被偵測到,且不
受其它菌屬,例如大腸桿菌非專一性的干擾。將此探針應用於菌血症病人
血液篩選時,發現準確性不如預期理想,僅達50%,推測不同抗體來源所
製成之探針及固定化技巧的好壞是影響檢測結果的原因所在。(三)、心
臟藥物digoxin微量分析 以Anti-digoxin monoclonal antibody固定晶片
為探針,直接分析試液中digoxin的含量,測量極限只達mg/mL之範圍。為
提高測量靈敏度,改以BSA-digoxin conjugate 固定晶片為探針,利用加
入定量的anti-digoxin monoclonal antibody 與free digoxin,使BSA-
digoxin conjugate 與free digoxin 競爭Anti-digoxin monoclonal
antibody結合位置,以間接測定試液中 digoxin 的濃度。結果顯示,利
用競爭性間接測量時,測量digoxin濃度可低至1.04ng/mL,符合臨床偵測
血液中digoxin濃度之需求。
The advantages of piezoelectric crystal immunosensor are their
high sensitivity, high selectivity, real-time analysis, low
cost, and fast response. These properties are useful in a
clinical diagnosis to accelerate the process of examination and
to provide fast and accurate information to a doctor. In the
present study, we use piezoelectric crystal immunosensor to
develop three fast procedures to detect anti-Helicobacter pylori
antibodies, Staphylococcus aureus and digoxin.1.Detection of
anti-H. pylori antibodies Proteins extracted from H. pylori are
immobilized on the crystal to detect the existence of anti-H.
pylori antibodies in the host serum. The results revealed that
when serum was diluted in a range of 1/70 ~ 1/500, the decrease
frequency of the crystal was correlated to the concentration of
serum. The decrease may be due to the liquid viscosity and the
interference of nonspecific protein binding. The optimal
condition is obtained when the serum was 70-fold dilution and
the sample volume applied to the system was 250 mL. In this
condition, H. pylori infected serum and normal serum result in a
100Hz difference of frequency decrease of the crystal. In
addition, the crystal was regenerated after washing with 1 M
NaOH.2. Detection of S. aureus in serumAnti-protein A antibody,
IgG or human plasma are immobilized on the crystal for detecting
S. aureus. Result showed that immobilized anti-protein A
antibody was an appropriate probe for S. aureus detects.
Frequency decrease was measured as low as bacterial density of
2.14 x 105 cells/mL. However, when blood culture sample from
infected patients was appliedin this system, the specificity and
sensitivity were low. We found that the anti-protein A
antibodies from various commercial sources severely influenced
the test accuracy.3. Analysis of digoxin concentrationBy using
with immobilized anti-digoxin monoclonal antibody as a probe to
detect digoxin concentration in sample did not provide a
sufficient sensitivity. To overcome this issue, a competitive
method with BSA-digoxin as a probe was used. By adding a certain
amount of monoclonal anti-digoxin antibody to the unknown
sample, we could indirectly measure the concentration of digoxin
that compete by bound to immobilized BSA-digoxin. As low as 1.04
ng/mL of digoxin could be detected by this indirect method.
QRCODE
 
 
 
 
 
                                                                                                                                                                                                                                                                                                                                                                                                               
第一頁 上一頁 下一頁 最後一頁 top
系統版面圖檔 系統版面圖檔