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研究生:陳愫蘭
論文名稱:人類卵巢畸胎瘤鱗狀上皮細胞C9由低氧引起錳超氧歧化酶的表現
論文名稱(外文):Hypoxia Induced Manganese Superoxide Dismutase in C9 Cells
指導教授:趙壯飛俞克柔
學位類別:碩士
校院名稱:國防醫學院
系所名稱:生物及解剖學研究所
學門:生命科學學門
學類:生物訊息學類
論文種類:學術論文
論文出版年:1998
畢業學年度:86
語文別:中文
論文頁數:46
外文關鍵詞:MnSODKeratinocyte Differentiation
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  含錳的超氧歧化□(manganese superoxide dismutase; MnSOD),是位於粒線體內基質且由細胞核製造的酵素,負責催化:O2-+O2-+2H+→H2O2+O2反應,使超氧負離子能歧化成二氧化氫和氧。細胞的分化可能會導致氧的游離基產生。本實驗室之前的研究則顯示:當角質細胞在走向完全分化的過程中,其角質素K19和角質素K14會有抑低調控的現象。而在人類卵巢癌C9細胞以TPA處理培養後,可造成K14類似在體內分化過程所發生的抑低調控,另外,當以低氧環境培養C9細胞或以鎳離子處理時,均使得K19發生抑低調控。而這些現象似乎都和氧的代謝有關,因此我們想探討是否MnSOD的表現和K19及K14的抑低調控,在角質細胞分化的過程中存在著相關性。分別以3%低氧培養、100n g/ml TPA、0.6nM鎳離子處理C9細胞後,以免疫螢光染色觀察,生化方法分析MnSOD的活性,和以反轉錄-聚合□鏈鎖反應(RT-PCR)分析MnSOD mRNA,發現經過低氧、鎳離子、TPA、處理都可刺激MnSOD 在C9細胞的表現,這些結果顯示在MnSOD的表現和角質細胞分化的過程之間存在著相關性。
  Manganese superoxide dismutase (MnSOD), a nuclear encoded mitochondrial matrix enzyme, can catalyzes the reaction:O2-+O2-+2H+→H2O2+O2.It has been shown that the over production of reactive oxygen species was associated with cell differentiation. According to our previous works contrarily showed that Keratin 19 (K19) and 14(K14) will be down regulated when keratinocytes go on the journey to terminal differentiation. When HOTC C9 cells treated with TPA, the K14 will be down regulated. When cell cultured under the hypoxia or treated with nickel chloride, the K19 was down regulated under those conditions. To further investigate the correlation of expression of MnSOD and K14 and K19 down regulation during keratinocyte differentiation. The distribution of MnSOD in C9 tumor growth in nude mice were examined by immunohistochemistry. It demonstrated that the MnSOD expression was anti-parallel correlated with the expression of K19. When C9 cells treated with TPA, hypoxia, and nickel chloride, the MnSOD were induced in time dependent manner by using immuno-histochemical biochemical enzyme activity assay and RT-PCR respectively. The results showed the MnSOD in C9 cells can be stimulated by TPA, hypoxia and nickel chloride treatment. It conclude that the MnSOD expression may involve in the process of keratinocyte differentiation.
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