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研究生:何惠玟
論文名稱:飼料中磷脂膽鹼質與量對幼蟳成長和組織中脂肪酸的影響
論文名稱(外文):The Qualotative and Quantitative Effect of Dietary Phosphatidylholine on Growth and Tissue Fatty Acids of Juvenile Scylla Serrata
指導教授:沈士新沈士新引用關係
學位類別:碩士
校院名稱:國立海洋大學
系所名稱:水產養殖學系
學門:農業科學學門
學類:漁業學類
論文種類:學術論文
論文出版年:1998
畢業學年度:86
語文別:中文
論文頁數:77
中文關鍵詞:氯化膽鹼幼蟳
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  本論文探討磷脂膽鹼質與量的添加對幼蟳(Scylla serrata)成長和組織中脂肪酸的影響。實驗I以4% cod liver oil 作為基礎油脂再分別添加1、2、3、4%的磷脂膽鹼(fresh egg phosphatidylcholine, FPC)做為處理組,另外一組則添加2.4%氯化膽鹼(choline chloride),控制組添加4% cod liver oil餵予重約60 mg的幼蟳。八週食實驗,蟳食飼料添加4%的FPC成長最好,其增重率為2766%,控制組之蟳增重率最差1731%,餵食2.4% choline chloride 蟳體的增重率為1915%,幼蟳食1、2、3、4% FPC的增重率沒有顯著差異。蟳餵食含有2.4% choline chloride蟳體增重率較餵食2、3、4% FPC低。蟳體脫殼次數不會因為餵食FPC而增加。活存率以控制組活存率較低,而餵食添加3%以上FPC或是餵食添加2.4% choline chloride幼蟳活存率為100%。蟳餵食FPC其體極性脂質有較高的n-3高度不飽和脂肪酸的蓄積量,人由蟳體測得磷脂鹼含量,推測幼蟳可藉由膽鹼合成磷脂膽膽鹼,然合成速度太慢無法滿足幼蟳成長的需求,因此磷脂膽鹼在飼料中的添加實為必要。
  實驗II以3% cod liver oil作為基礎油脂再分別添加1%大豆磷脂膽鹼(soybean phosphatidylcholine, SPC20% purity)、1%大豆磷脂膽鹼(soybean phosphatidylcholine, SPC 40% purity)、1%雞蛋磷脂膽鹼(fresh egg phosphatidylcholine, FPC 60% purity)、1%液態大豆卵磷脂(fluid soybean lecithin, FSL15% purity)、1%去油大豆卵磷脂(deoil soybean lecithin, DSL 23% purity),控制組為4% cod liver oil。八週實驗結果顯示蟳餵食含1%FSL對幼蟳成長效果最好增重率為2709%成長顯著高於控制組之蟳,餵食FPC之蟳增重率為2257%,其它處理組的增重率(SPC, DSL)為2309~2472%。幼蟳活存率以控制組最低只有80%,餵食PC的處理組則有87~100%活存率。蟳體脫殼次數並沒有因為餵食PC而增加。實驗結束後蟳體膽固醇和磷脂膽鹼含量以餵食有PC的蟳高於控制組。
  This study investigated the qualitative and quantitative effects of dietary phosphatidylcholine on the growth and tissue fatty acids of juvenile Scylla serrata. In experiment 1,4% cod liver oil was used as lipid source. Diets containing 0, 1, 2, 3, and 4% chicken phosphatidylcholine, and 2.4% choline chloride were evaluated. Crabs fed diets containing 1, 2, 3, and 4% phosphatidylcholine had significantly higher weight gain than those fed diet without supplemented phosphatidylcholine. Crabs fed diet supplemented with 2.4% choline chloride had lower weight gain than those fed diets containing 2, 3, 4% phosphatidylcholine. The survival rate ranged from 80% to 100%. Crabs mean molting frequency were not increased when they were fed diets containing phosphatidylcholine. Crabs fed diets containing phosphatidylcholine had higher polar lipid fraction of n-3 HUFA retention.
  The effect of different phosphatidylcholine sources on the growth rate of S. serrata were investigated in experiment II. The treatment diets containing 3% cod liver oil were included with 1% of SPC (soybean phosphatidylcholine, 20% purity), 1% of SPC(soybean phosphatidylcholine, 40% purity), 1% of FPC(fresh egg phosphatidylcholine, 60% purity), 1% of FSL(fluid soybean lecithin, 15% purity), 1% of DSL (deoil soybean lecithin, 23% purity). The diet including 4% cod liver oil was used as control diet. Crabs fed diet containing FSL had the highest weight gain than those fed other treatments. Crabs fed diets supplemented with different kinds of phosphatidylcholine had higher lever of cholesterol and phosphatidylcholine than that those fed diet supplemented without phosphatidylcholine. The survival rate ranged from 80% to 100%.
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